New Immunoaffinity Kits Enables Fast, Simple and Highly Reproducible Protein Digestions

18 Oct 2016
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

The new Thermo Scientific SMART Digest Immunoaffinity (IA) Kits deliver fast, simple and highly reproducible digestion of proteins for characterization and quantification applications.

Researchers undertaking quantitation studies typically increase sensitivity by purifying low level proteins from complex biological matrices, followed by protein digestion. The SMART Digest Immunoaffinity Kit design combines immunocapture and the digestion process in a single well. This simple and easy approach not only increases reproducibility but also significantly decreases sample preparation time from overnight to a few hours. Enabling laboratories using the kit to increase sample throughput and quality of results while receiving improved return of investment. The key application the kit is intended for is the quantification of biomarkers in bioanlaysis and biopharma.

Features:

  • Increased productivity- significant increase in sample throughput can be achieved compared to a standard protein quantification sample preparation process
  • Improved cost efficiencies- increased sample quality and throughput deliver an improved return of investment to laboratories
  • Greater usability- simple and easy approach for immunocapture and protein digestion for quantitation studies

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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.
New Immunoaffinity Kits Enables Fast, Simple and Highly Reproducible Protein Digestions