New High Resolution Image Analyser Generates Excellent Results with Qdot Labelled Western Blots

10 May 2007

Product news

Syngene, a world-leading manufacturer of image analysis solutions, today introduced G:BOX Chemi XL, its new automated image analyser, ideal for producing precise images of Western blots labelled with any Qdot® colour.

The new G:BOX Chemi XL features a high resolution (5.5 mega pixel, digital zoom), 16 bit CCD cooled camera inside a darkroom fitted with motor driven sample stage. By adding Syngene’s 9-position filter wheel, lighting unit and Qdot filter options, the system can provide fast, accurate imaging of multiple Qdot antibodies on the same blot without the need for re-probing the sample. These features combined with the camera’s ability to separate close, different coloured band images makes the G:BOX Chemi XL an intelligent choice for scientists that need a reliable method of generating images of Qdot labelled Western blots.

The G:BOX Chemi XL comes with GeneTools, easy to use image analysis software and a new version of GeneSnap image capture software, which saves time with its new focus indicator and digital zooming features.

Laura Sullivan, Syngene’s Divisional Manager commented: “A growing number of researchers are using Qdots to label Westerns because they can tag their proteins with different coloured fluorescent markers on one blot, so it is easier for them to differentiate total and post-translationally expressed proteins. However, many have found to accurately capture this type of multiplex image you need an imager which can visualise different fluorescent wavelengths, yet has exceptional resolving power.”

Laura Sullivan concluded: “This is why we are delighted to add the G:BOX Chemi XL to our range because it will help scientists overcome these difficult imaging challenges. As well as being able to separate different Qdot colours from each other, the system’s peltier cooled camera also allows chemiluminescence imaging and means the G:BOX Chemi XL is an essential tool where accurate imaging of fluorescent and chemiluminescent Western blots is critical to the research.”

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
New High Resolution Image Analyser Generates Excellent Results with Qdot Labelled Western Blots