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New help for protein researchers on Malvern website

Malvern Panalytical
30 Aug 2006

Product news

A new interactive function on the Malvern Instruments website allows researchers to quickly determine the minimum sample concentration required for measurements of their protein with the company’s Zetasizer Nano systems.

Users simply need to enter the molecular weight of their biomolecule into the Zetasizer Nano Minimum Concentration Calculator. Separate sections on the calculator cover sizing, zeta potential and molecular weight determinations.

Malvern’s Zetasizer Nano is now the most widely used light scattering system for the size measurement of particles and molecules in solution. It is designed specifically to meet the low sample volume and high sensitivity requirements essential for protein characterization.

Easy to use and measuring molecules as small as 0.3 nm hydrodynamic radius, the Zetasizer Nano offers the high sensitivity required for the measurement of solutions of proteins. Applications include protein size and molecular weight measurement, screening for protein crystallization conditions, determining aggregates, and measuring melting points. Furthermore, size trends as a function of pH or additive concentration, for example, can be automated by coupling it to the MPT-2 autotitrator.

To use the calculator, visit the article webpage.

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Malvern PanalyticalCompany website

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.

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