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New Gene Expression Kit for Multiplex Toxicity Screening

Beckman Coulter Diagnostics
27 Jun 2007

Product news

Beckman Coulter, Inc. has introduced the GenomeLab™ GeXP Rat MultitoxPlex Kit for preclinical drug research screening. The new kit for the GenomeLab GeXP Genetic Analysis System contains 25 genes involved in key toxicological pathways including toxicity, stress, DNA damage and apoptosis. It also includes three reference genes and one internal standard.

The GeXP Rat MultitoxPlex Kit and the GeXP System deliver multiplexed quantitative analysis of genes from hundreds of samples at a time, providing higher throughput than real-time PCR* along with important cost savings. GeXP Rat MultitoxPlex assays run in 96-well plate format, providing a quick way to screen large compound libraries. Researchers may also add their own genes of interest to the new Rat MultitoxPlex panel. The GeXP system analyses up to 30 genes in one reaction.

The GeXP Rat MultitoxPlex Kit contains a control RNA that verifies the accuracy of the assay. The kit is used in conjunction with the GenomeLab GeXP Start Kit, which contains all the necessary buffers and reagents to carry out the assay. The GeXP kits are for research use only.

“Many pharmaceutical companies are currently conducting preclinical toxicity studies looking at one gene at a time, using real-time PCR,” explained Zhiming Jiang, Ph.D., product manager for Beckman Coulter. “The new GenomeLab GeXP Rat MultiToxPlex Kit measures 25 genes in one reaction. This is just one more example of how Beckman Coulter can simplify and automate lab processes to bring significant benefits to the researcher.”

The GeXP technology was developed to fill the gap between whole genome microarrays and single-gene real-time PCR. With Beckman Coulter’s automation capabilities and validated methods, gene expression sample preparation, quantitation and normalisation and reaction setup can be carried out on the Biomek® 3000 liquid handler using the Agencourt® RNAdvance™ chemistry. When used together, the Biomek 3000 workstation, validated methods and the GeXP Genetic Analysis system deliver a complete solution for high-throughput quantitative gene expression analysis, from sample to answer within eight hours.

*The PCR process is covered by patents owned by Roche Molecular Systems and F. Hoffman-La Roche Ltd.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microarray AnalysisMicroarrays, also known as biochips, are used for the detection and analysis of multiple genes, proteins, antibodies, or biomarkers on a single microchip. This can reveal information on protein or gene expression, single nucleotide polymorphism (SNP), copy number variation (CNV), epigenetics and patient health in clinical diagnostic tests. Discover a range of microarray scanners and prefabricated antibody, protein, RNA and DNA microarrays for your analysis or consider creating your own custom microarrays with a microarray printer. Find the best microarray products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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