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New Gene Cloning Tool Is a Safer, More Sensitive Option

Cleaver Scientific Ltd
1 Aug 2012
Tesni Perry
Administrator / Office Personnel

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A new, ready-to-use, safe, sensitive and more flexible alternative to ethidium bromide and other DNA stains has been introduced by electrophoresis specialists CLEAVER SCIENTIFIC (CSL). RunSafe™ is a significant new tool for gene cloning applications which enables scientists to see their samples separate in real time.

RunSafe™ is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of gels. It is easy to use. Simply mix, load and run and it acts as both a loading dye and a DNA stain combined for added speed and convenience for optimum economy and low environmental impact. No expensive waste management is required and it complies with Clean Water Act standards.

Supplied in Cleaver Scientific’s 6x DNA loading buffer, RunSafe™ can be used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It is a highly sensitive stain available for detecting double-stranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF and Orange G) for visual tracking of DNA migration during electrophoresis.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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New Gene Cloning Tool Is a Safer, More Sensitive Option