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New Gel System for High Quality Protein Electrophoresis

Cytiva
5 Sept 2011
Roger Wayman
Administrator / Office Personnel

Product news

GE Healthcare’s horizontal Amersham ECL Gel System is designed for high quality protein electrophoresis enabling efficient, high-quality and reproducible protein separation. Offering high sensitivity and resolution, it works with standard protein detection protocols and is easy to integrate into the Amersham ECL Western blotting workflow.

The core of the system is Amersham ECL Gel, a precast gel available in a variety of concentrations and well configurations. Gels are processed using Amersham ECL Gel Box, a dedicated electrophoresis unit with horizontal design, and significantly increased ease of use compared to vertical systems.

Everything required to access and cut the gel is included in the cassette. At 1.4 mm thickness, it is thicker than many other precast and hand-cast gels, providing peace-of-mind when handling and reducing the risk of gel breakage in downstream processing.

The Amersham ECL Gel System aims to make PAGE as easy as gel DNA electrophoresis. The horizontal format allows for easier sample application, gives a full view of the entire electrophoresis run and requires virtually no assembly, reducing preparation time. The gel contains no SDS, making it appropriate for protein analysis under native and denaturing conditions, depending on the choice of running and sample loading buffers. Coupled with lower buffer consumption and no risk of leakage between buffer chambers, the gel system allows the resolution of complex samples.

Amersham ECL Gel is compatible with standard Laemmli buffers and has a shelf life of up to 12-months, without any compromise in performance. For added convenience, a 10x concentrated running buffer for SDS-PAGE is available, as well as pre-cut Hybond™ membranes for Western blotting applications.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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New Gel System for High Quality Protein Electrophoresis