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New Eppendorf µCuvette G1.0 Allows Life Scientists to Analyze Even Smaller Sample Volumes

Eppendorf
29 Oct 2012

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Eppendorf has introduced the new Eppendorf µCuvette G1.0™, designed for the accurate and reliable measurement of small volumes of highly concentrated biomolecules, such as proteins and nucleic acids. Users can work easily with samples as small as 1.5 μL without the need for a time-consuming dilution step.

The µCuvette G1.0 expands the measuring range of the Eppendorf BioPhotometer and the Eppendorf BioSpectrometer. Synchronized apertures mean that a closed system is created when used alongside Eppendorf detection instruments, for optimum results and reliable operation.

“Photometric measurements are only as accurate as the device and corresponding accessory,” said Dr. Tanja Musiol, Global Product Manager Detection at Eppendorf AG. “Selecting the perfect cuvette is just as important as choosing the right device. Higher sample concentrations require a shorter optical path length for measurement. The optical path length of the μCuvette G1.0 is just 1 mm, ten times shorter than the light path length of standard cuvettes. This provides a better system for measurements at high concentrations.”

Eppendorf has a worldwide reputation as producers of premium laboratory equipment. The µCuvette G1.0 expands the company’s extensive range of products for micro-volume applications.

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µCuvette G1.0™

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The Eppendorf µCuvette G1.0 is a microliter measuring cell, and therefore it is especially suited for measuring high concentrations. The higher the concentration of a sample, the shorter the optical path length should be with which the sample will be measured. Thanks to a special Eppendorf coating procedure, the sample volume is positioned exactly and the drop will be shaped with precision. This technology makes it possible to fix the drop without producing any pronounced edges or projections. This minimizes the risk of sample carry-over and simplifies to a large extent the cleaning of the sample surface. Due to the used materials and the selected measuring principle, the self absorption of the µCuvette G1.0 is very low and can be compared to that of standard quartz glass cuvettes. The µCuvette G1.0 has been developed exclusively for Eppendorf BioPhotometer and BioSpectrometer. The devices are pre-programmed to a layer thickness of 1mm. The software realizes all calculations considering the layer thickness. In addition, the optical characteristics of the devices, as e.g. the light beam focussing and light beam guidance, are optimally adjusted to those of the µCuvette G1.0. This optimization as well as the connection of both cuvette parts by means of a low-backlash journal bearing joint provide for highly reproducible conditions during measurement. Application examples: • Concentration determination of nucleic acids and proteins • Measurement of high sample concentrations without dilution • Low sample volumes

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New Eppendorf µCuvette G1.0 Allows Life Scientists to Analyze Even Smaller Sample Volumes