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New England Biolabs Releases the NEBNext® Microbiome DNA Enrichment Kit

New England Biolabs Inc.
3 Jun 2013
Sarah Thomas
Associate Editor

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New England Biolabs (NEB) announces the release of the NEBNext® Microbiome DNA Enrichment Kit, which uses a novel method to separate microbial DNA from host DNA, thereby reducing the high cost of sequencing microbiome DNA to a more practical level.

Microbiome samples are commonly dominated by host DNA (up to 99%). This complicates the genetic analyses of these samples, particularly total microbiome DNA sequencing studies. Since only a small percentage of sequencing reads pertain to the microbes of interest, obtaining sufficient sequence coverage of the microbiome DNA can become cost-prohibitive or even technically infeasible.

The NEBNext Microbiome DNA Enrichment Kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, enabling quick and easy removal of the contaminating host DNA in about 30 minutes. The microbial DNA-enriched sample is then ready to be processed for multiple downstream applications, including next generation sequencing, qPCR and endpoint PCR.

“Although microbiome whole genome sequencing provides more information than other methods, such as 16S analysis, this method has proven difficult for many samples, due to the presence of contaminating host genomic DNA,” says Fiona Stewart, Product Marketing Manager, Next Generation Sequencing at NEB. “The NEBNext Microbiome DNA Enrichment Kit makes it possible to substantially enrich samples for non-host, microbial DNA, while retaining microbial diversity, thereby improving the quality and cost-effectiveness of downstream analyses.”

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NEBNext® Reagents for DNA Library Preparation

New England Biolabs Inc.

NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Preparation: Convenient formats – Enzymes, buffers and nucleotides are included, at appropriate concentrations and in appropriate volumes, and are available in set or master mix format. Large and small sizes are available. Functional Validation – Each set is validated together by preparation of a library from an accepted RNA reference sample, that is sequenced using an Illumina GAIIx. Stringent Quality Controls Value Pricing Novel Protocol for small RNA - Higher yield and substantially reduced adaptor dimer formation suitable for methylated and unmethylated small RNAs. Applications of NEBNext Reagents for RNA Library Preparation: mRNA library preparation for next generation sequencing Small RNA library preparation for sequencing with Illumina and SOLiD Expression library construction

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Genome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Bacterial Genome AssemblyMicrobiomeMicrobiome research focuses on the complex communities of microorganisms living in and on humans, animals, plants, and environments. It plays a crucial role in health, disease, and ecological balance. Explore tools and resources for microbiome analysis, including sequencing platforms and bioinformatics software.

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New England Biolabs Releases the NEBNext® Microbiome DNA Enrichment Kit