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New Dynabeads® SAX Added to Advanced Magnetic Bead Chromatography Product Line

Invitrogen Limited
5 Sept 2006
Todd Wielgos
Research Director / Manager

Product news

Dynal, now part of Invitrogen Corporation, today announced the release of a new product in its extensive proteomic sample preparation range; Dynabeads® SAX.

Based on strong anion exchange (SAX), these magnetic beads can fractionate protein and peptide samples quickly and easily based on differences in pI and net charge, for downstream analyses such as mass spectrometry. This adds further functionality to the existing magnetic bead chromatography product line launched last year; Dynabeads® WCX, Dynabeads® RPC Protein and Dynabeads® RPC 18.

These products offer an excellent alternative to chromatography columns, spin columns and pipette tip technologies. Dynabeads® SAX are designed with an optimised surface for binding and fractionation of intricate protein and peptide mixtures. The magnetic separation methodology is very fast, highly reproducible, flexible and scalable, and can be automated on a wide variety of liquid handling platforms.

According to Business Area Manager, Beate Rygg Johnsen, the enlarged magnetic bead chromatography product range offers even more options to simplify the reduction of sample complexity. “These versatile Dynabeads® products can be used to concentrate, prepare and desalt complex protein and peptide mixtures and for biomarker discovery. Both top-down and bottom-up approaches can be employed. Acting as a controllable solid phase support, the beads respond to a magnetic field allowing bound material to be precisely separated from salts and other contaminants within minutes. Proteins or peptides are then eluted off in one or more fractions, e.g. by step-wise elution. Beads with different surface characteristics can be combined to isolate different fractions from the same sample. This means that more information can be obtained and the sample is efficiently and reproducibly prepared for further downstream mass spectrum analysis, HPLC or 1D/2D gel electrophoresis.”

All Dynabeads® are engineered to provide uniform spheres with even magnetic properties and maximum surface area. They remain dispersed in solution but very quickly move in response to a magnetic field, with no magnetic remanence (superparamagnetic). The different beads available have been designed to accommodate many different surface chemistries and are available in different sizes to suit different proteomic, genomic and in vitro diagnostic applications.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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New Dynabeads® SAX Added to Advanced Magnetic Bead Chromatography Product Line