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New Customized Service for the Generation of Stable Cell Lines Expressing Specific Proteins of Interest

Dualsystems Biotech AG
7 Jul 2010
Sarah Sarah
Marketing / Sales

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Dualsystems Biotech AG now offer a stable cell line generation service. This two part process offers a wide selection of tags and takes just six weeks to complete. Available cell lines include HEK293 (human embryonic kidney cells), INS-1 (beta pancreatic islet cells), CHO (Chinese hamster ovary cells) and 3T3 (primary mouse embryonic fibroblast cells) along with many others.

The first part of the process consists of the construction of an integration vector containing the gene of interest and its transfection into the appropriate cell line. Tags can be placed at either the N- or C-terminus. Once a stable cell line has been selected, clones are expanded and expression of the protein is verified by Western blotting. Upon completion of the process, Dualsystems delivers five vials of frozen stocks with at least five million cells per vial, a Western blot demonstrating protein expression, and the cell cultivation protocol.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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