New Controls for PCR, RNA Microarray & Antibody Array Studies

15 Dec 2010
Kerry Parker
CEO

Product news

The AMSBIO range of Universal Reference Products (RNA, cDNA and Protein Lysate) has been expanded to include standards for studies involving humans as well as for animal species including Monkey, Dog, Mouse, Rat, and Chicken. These products provide ideal controls for high throughput PCR, RNA microarray and antibody array studies.

All AMSBIO Universal Reference Products are derived from very diverse tissue sets (from both male and female donors), and manufactured on an industrial scale, to ensure the broadest coverage of genes and to minimize the lot-to-lot variation.

Universal RNA from AMSBIO is total RNA isolated from sources, by modified guanidine thiocyanate techniques. Universal RNA can be used as controls for optimizing and trobleshooting single and two colour microarray studies. Universal cDNA is prepared by reverse transcribing AMSBIO's Universal RNA by random hexamer or oligo dT primer. Total RNA is isolated by modified guanidine thiocyanate techniques. Universal cDNA from AMSBIO can be used as controls for real time PCR and regular PCR, and also as gene pools for cloning genes. Universal Protein Lysate from AMSBIO is total protein isolated from whole tissue sources. Universal Protein Lysate can be used as controls for comparison of protein expression and antibody microarray studies.

For further information on Universal Reference products please visit the article webpage.

Links

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.
New Controls for PCR, RNA Microarray & Antibody Array Studies