SelectScience
Join Free
Accelerating Science
NewsSeparations

New Columns Accelerate LC/MS Analysis of Synthetic Oligonucleotides

Phenomenex Inc
20 Apr 2010
Sarah Sarah
Marketing / Sales

Product news

Phenomenex Inc. introduces Clarity® Oligo-MS™ columns for rapid and efficient LC/MS characterization and quality control of synthetic RNA and DNA. Based on the company’s core-shell particle technology, the new C18 columns deliver significantly shorter run times than traditional media, along with increased resolution and sensitivity. With the high resolving power of Clarity Oligo-MS, impurities in complex synthetic mixtures can be separated from the peak of interest in less than 10 minutes.

These new Oligo-MS columns, which are part of the Clarity BioSolutions portfolio, are packed with either 2.6µm or 1.7µm particles, enabling high efficiency on any HPLC or UHPLC platform. The Clarity Oligo-MS 2.6µm columns operate at reduced backpressures compared to other oligonucleotide-specific columns, delivering UHPLC performance on any LC instrument, and methods are easily transferable. The Clarity Oligo-MS 1.7µm columns boost the performance of existing sub-2µm methods on high-pressure systems.

“LC/MS is the analysis technique of choice in synthetic oligo quality control as well as for the characterization of oligo-based therapeutics and their metabolites,” explained Cathy Cordova, marketing manager for Phenomenex. “With our new core-shell particle technology, we bring significant improvements to this process in the areas of sample throughput and MS sensitivity.” Phenomenex maintains a dedicated team of R&D, marketing, sales and service experts to support its Clarity BioSolutions product line for synthetic oligonucleotide purification and characterization.

With traditional, fully porous particles, efficiency decreases as flow rate increases, resulting in loss of resolution and sensitivity and longer overall analysis time. The Phenomenex core-shell technology enables high resolution and sensitivity over an extended linear velocity without generating excessive backpressure. These columns provide roughly twice the efficiency of fully porous 3-micron columns and three times the efficiency of fully porous 5-micron columns, with significantly lower limits of detection and quantitation.

Links

Phenomenex IncCompany website

Tags

LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

Related articles

New Columns Accelerate LC/MS Analysis of Synthetic Oligonucleotides