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New Clinical PCR Master Mix Developed for Challenging Genotyping, CNV Experiments

Thermo Fisher Scientific

Applied Biosystems TaqPath ProAmp PCR Master Mix formulated to maximize the specificity and reproducibility of inhibited real-time PCR samples

15 Sept 2016
Alex Waite
Editorial Assistant

Product news

Thermo Fisher Scientific continues to create products that meet the changing needs of clinical real-time PCR (qPCR) customers by launching Applied Biosystems TaqPath ProAmp PCR Master Mix.

Applied Biosystems™ TaqPath™ ProAmp™ Master Mixes are versatile master mixes developed for high-throughput genotyping and copy number variation (CNV) protocols requiring accurate results from samples containing PCR inhibitors. TaqPath ProAmp Master Mixes are designed to deliver sensitive and reproducible results from genomic DNA targets.

Two formulations of this Applied Biosystems multiplex assay-compatible master mix are available: TaqPath ProAmp Multiplex Master Mix with Mustang Purple for multiplexing up to four targets; and TaqPath ProAmp Master Mix, which includes ROX™ as the passive reference dye. Both mixes enable measurement of FAM™, VIC™, and ABY™ dye or other dyes with similar emission wavelengths. The multiplex version also allows the use of JUN™ dye, or other dyes with similar emission wavelengths, in the channel typically used to measure ROX dye for single nucleotide polymorphism (SNP) genotyping and other higher multiplex applications.

These two formulations join the line of Applied Biosystems TaqPath General Purpose Reagents—qPCR and RTqPCR—master mixes manufactured in an ISO 13485 certified facility to help ensure manufacturing excellent manufacturing consistency. 1Dynamic range is a property of both the assay and template concentration in the sample, as well as in the master mix, thus individual results may vary.

Features:

  • High data quality--high specificity, dynamic range1, and reproducibility for genotyping and copy number determination
  • Tolerance to inhibitors--compatible with samples prepared from human or animal sources (buccal swabs, blood, and card punches)
  • 72-hour pre-PCR benchtop stability--proprietary Dual-Lock™ Taq DNA Polymerase hot-start mechanism for setup on automated workflows
  • Multiplexing breadth—Detect up to 4 targets per reaction
  • Manufacturing consistency—manufactured in an ISO 13485-certified facility

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HematologyIn Haematology / Hematology, complete blood cell counts (or full blood counts) are obtained using automated blood count analyzers to enumerate blood cell types.  Hematology also encompasses haemostasis and coagulation, thrombophilia and hemophilia, plasma viscosity and ESR analysis, hemoglobinopathies, cell morphology and haematinic measurement.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Real-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.GenotypingGenome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.Copy Number Variation

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