New CFX Qualification Plates from Bio-Rad for PCR Performance Verification

Bio-Rad Laboratories, Inc. today announced the launch of the CFX qualification plate, an easy-to-use tool for accurately verifying the performance of any CFX real-time PCR system, providing researchers with confidence in their results.

Preloaded with an optimized master mix containing Bio-Rad’s SsoAdvanced™ SYBR® Green supermix, the CFX qualification plate provides a five-point standard curve, two unknown groups, and no-template controls, allowing for the rapid determination of a pass/fail instrument performance result.

Included in Bio-Rad’s CFX Manager™ software is the new qualification plate check tool for analyzing the CFX qualification plate data. The tool uses an algorithm to determine a pass/fail performance result based on two criteria: twofold discrimination with a 99.7% confidence level and single-product melt peaks for the two unknowns. A passing result indicates good optical performance and uniform thermal block control.

The CFX qualification plate joins Bio-Rad’s new PCR instrument validation tools for verifying Bio-Rad’s real-time PCR detection systems.