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New amaxa Stem Cell Kits Provide Transfection Efficiencies of up to 80%

Lonza Group Ltd
25 Jun 2008

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Embryonic stem cells are powerful tools for discovering developmental processes at the cellular level. Hence, amaxa introduces four new Human Stem Cell Nucleofector Kits, for single reactions and up to 96-well transfections, suitable for various stem cell lines. With these kits, transfection efficiencies of 60% up to 80% can be achieved for stem cells, combined with high viability.

Apart from embryonic stem cells, amaxa's Stem Cell Nuclefoector Kits enable researchers to efficiently transfect various types of stem cells such as mesenchymal, hematopoietic or neural stem cells, without compromising potential for differentiation. Optimized transfection conditions allow the use of DNA, siRNA or peptides which make the non-viral method a flexible tool to assist scientists in their individual research, e.g. study differentiation patterns or characterizing the basic metabolic processes of stem cells.

To find out more about the efficient transfection of stem cells download the flyer 'Nucleofector Technology in Stem Cell Research' please contact amaxa.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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New amaxa Stem Cell Kits Provide Transfection Efficiencies of up to 80%