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Multiplex Immunohistochemical (IHC) Analysis from Enzo Life Sciences, Inc.

Enzo Life Sciences
1 Jul 2013

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Continuing our position as a leader in detection technologies, Enzo Life Sciences offers a convenient set of tools for single-color and multi-color immunohistochemical (IHC) analysis, featuring MultiView™ multiplexing kits, PolyView™ reagents, and the market’s broadest portfolio of HighDef™ Chromogens. Combined with over 1000 IHC validated antibodies and a complete set of ancillaries, Enzo offers all the tools to run an efficient IHC assay from start to finish.

MultiView™ detection kits offer a complete solution for multiplex IHC, providing increased efficiency, reduced reagent use, and preservation of precious tissue samples. Each kit includes biotin-free nanopolymer-based PolyView™ detection reagents to reduce endogenous biotin-associated background, HighDef™ chromogens for crisp two-color development, as well as buffers and blocking reagents. The flexibility of PolyView™ reagents and MultiView™ kits simplifies custom multiplex protocols to enable up to four-color staining within the same tissue sample. Our industry-leading palette of HighDef™ chromogens provides the most choice in color options, featuring HighDef™ Yellow HRP, and including multiple horseradish peroxidase (HRP) and alkaline phosphatase (AP) options. The portfolio also includes streptavidin-based SAView™ detection kits for improved sensitivity and background compared to ABC detection protocols, also compatible with HighDef™ chromogens.

Our growing catalog of over 1000 IHC validated antibodies includes those for the detection of key signaling proteins, cell surface markers, cytoskeletal proteins, protein expression & folding, cell death, and more. Every Enzo antibody is backed by our Worry-Free Trial Program and expert technical support. With a broad selection of ancillary reagents for antigen retrieval, wash buffers, antibody diluents, and HighDef™ mounting medium, Enzo can help streamline simple and complex IHC assays from retrieval to coverslip.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Image AnalysisImage analysis involves the extraction of meaningful information from images, often using software to quantify and interpret visual data. It is widely used in cell biology, material science, and diagnostics. Increasingly, AI is being used to streamline image analysis. Explore image analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Multiplex Immunohistochemical (IHC) Analysis from Enzo Life Sciences, Inc.