ResourceLife Sciences
Multiomic 5-letter sequencing maps DNA modifications and genetic mutations in breast cancer cells
24 Nov 2025Researchers in Dr Sam Aparicio’s group at the British Columbia Cancer Research Centre (BCCRC) and the University of British Columbia, BC, Canada, utilised 5-letter sequencing technology, duet multiomics solution +modC, to investigate ‘epigenetic rewiring’ in breast cancer cells.
In this study, duet multiomics solution +modC helped to reveal:
- the epigenetic landscape of untransformed diploid breast epithelial cells with wild-type, p53-/-RCA1-/- and p53-/-BRCA2-/- genetic backgrounds
- significant activation of stem cell enhancers through reduced DNA methylation in p53-/-BRCA1-/- cells only similar activation of stem cell enhancers in a triple negative breast cancer (TNBC) patient xenograft sample
- the epigenetic rewiring caused by BRCA1-/-, identifying it as a crucial gene for this type of cancer pathogenesis
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DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cancer ResearchCancer research aims to understand the mechanisms of cancer development and progression to improve prevention, diagnosis, and treatment. From molecular biology to clinical trials, research spans a wide range of disciplines, including immunotherapy, targeted therapies, and drug discovery. Explore the best cancer research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Breast CancerBreast cancer is a type of cancer that begins in the cells of the breast and can spread to other parts of the body. Early detection, accurate diagnosis, and targeted treatments are essential for improving patient outcomes. Browse our peer-reviewed product directory to find the best breast cancer research and diagnostic tools, compare products, check reviews, and get pricing directly from manufacturers.BRCA1EpigeneticsEpigenetics refers to heritable changes in gene expression. These changes are not caused by alterations to the DNA sequence. Three of the main systems responsible for the initiation of epigenetic change are DNA methylation, histone modification and non-coding RNA-associated gene silencing.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.