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Most Viewed Clinical Downloads of 2014

SelectScience
20 Nov 2014
Sonia Nicholas
Managing Editor and Clinical Lead

Editorial article

1. Five-Minute Vitamin D Analysis with Online Sample Cleanup
Sample preparation is generally the bottleneck in a laboratory’s analytical workflow. With online sample cleanup, sample turnaround time, robustness and data quality are significantly improved for the LC/MS/MS analysis of both vitamin D forms. This application note, from Agilent Technologies, describes a fast, sensitive, and selective LC/MS/MS method for quantitative analysis of 25-OH Vitamin D2 and D3 in serum and plasma.

2. A Practical Guide to High Resolution Melt Analysis Genotyping
HRM can be used to detect single-base sequence variations or discover unknown genetic mutations. This application note from Bio-Rad highlights the benefits and applications of HRM and discusses key considerations, such as instrumentation, sample preparation and experimental design.

3. Meeting Evolving Clinical Demands for Assay Performance: Advances in Acridinium Ester Technology on the ADVIA Centaur Systems
This white paper reviews the clinical and other benefits delivered by some of the latest acridinium ester (AE) dependent assays from Siemens Healthcare Diagnostics. The history of acridinium ester molecule evolution, reviewing specific details of AE properties, ongoing research findings, and modifications to the AE molecule that continue to drive critical advances in assay performance and diagnostic testing are also detailed.

4. Fast and Easy B Cell Isolation for More Sensitive CLL Assays
B-Cell Chronic Lymphocytic Leukemia (CLL) causes malignant cells to proliferate and accumulate in the bonemarrow and peripheral blood. Array-based comparative genomic hybridization (array-CGH) and array-based single-nucleotide polymorphism analysis (array-SNP) are increasingly common tools for profiling genetic abnormalities in CLL and other haematological malignancies. This white paper discusses the advantages of these assays over traditional techniques.

5. Determination of Sulphur in Nasal Spray and Na₂S • 9 H₂O
Sulphur has a positive influence on chronic diseases; it is anti-inflammatory and stimulates wound healing. In order to apply the agent on the affected areas, the use of spray bottles has been established. For ensuring an effective and safe dosage, the quality of raw materials, as well as the final products, have to be controlled regularly. In this application note from Analytik Jena, nasal sprays from different batches and the raw sulphuric salt (Na₂S • 9 H₂O) were examined for their sulphur content.

6. Solutions for Efficient Sample Management and Biobanking
Modern biobanks usually contain a vast number of samples, as well as a wealth of sample-related information. This application note provides an overview on the Cryo.s™ product line, including cryogenic storage tubes and racks.

7. Improved Extraction and LC/MS/MS Analysis of Barbiturates from Urine Samples Using Strata™-X-Drug N SPE and Kinetex® Core-Shell HPLC/UHPLC Columns
The Strata™-X-Drug N SPE sorbent from Phenomenex is shown to provide both time and solvent savings, which can be multiplied when screening several samples at once, making it faster and more profitable for forensic labs to screen barbiturate samples. By pairing this improved extraction with a sensitive LC/MS/MS method on a Kinetex 2.6 μm C18 core-shell HPLC column, labs can resolve all five barbiturates screened, including pentobarbital and amobarbital, which has proved difficult using older HPLC methods.

8. MammaPrint and BluePrint in Early Breast Cancer: Clinical Implications of Prognostic Stratification and Molecular Subtyping
This poster describes a study to compare tumor subtyping methods of clinical immunohistochemistry (IHC)/fluorescent in situ (FISH) hybridization, and MammaPrint and BluePrint, and to determine which method of subtyping most accurately predicts long term outcome.

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Blood TransfusionBlood Transfusion involves giving donor blood to a recipient patient. Screening is essential to avoid transfusion reactions. Blood banks use immunohematological techniques to determine rh and ABO blood group, and screen for antibodies using specific antisera. Blood Banks use Direct Coombs Tests (DCT) and Indirect Coombs Tests (IAT) to detect hemolysis and Haemolytic Disease of the Newborn (HDN).HematologyIn Haematology / Hematology, complete blood cell counts (or full blood counts) are obtained using automated blood count analyzers to enumerate blood cell types.  Hematology also encompasses haemostasis and coagulation, thrombophilia and hemophilia, plasma viscosity and ESR analysis, hemoglobinopathies, cell morphology and haematinic measurement.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Point-of-CarePoint-of-Care Testing (POCT) or Near Patient Testing (NPT) products are available for urine, blood and other clinical chemistry analyses. POCT includes: blood glucose testing, blood gas and electrolytes analysis, rapid coagulation testing (PT / INR), rapid cardiac markers diagnostics, drugs of abuse screening, urine strips testing, pregnancy testing, fecal occult blood analysis, food pathogens screening, hemoglobin diagnostics, infectious disease testing and cholesterol screening.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Clinical MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Embryology / IVFEmbryology refers to the development of an embryo resulting from the fertilization of the ovum, to fetus stage. In vitro fertilization (IVF) refers to the fertilization of the egg outside of the human body, and is an important treatment for infertility.

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