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Most Downloaded Methods of 2015

SelectScience

Have you downloaded them all?

15 Jan 2016
Lois Manton-O'Byrne, PhD
Executive Editor

Editorial article

Have you downloaded them all?

2015 was an exciting year for SelectScience® – we unveiled our brand new website in March and held the hugely successful Scientists’ Choice Awards®, including the first Reviewers’ Choice Award. When it came to scientific research, our scientific community was most interested in improving their technique – becoming pipetting experts and refining their laboratory practice. Read on to find out the 10 most downloaded methods on SelectScience.net in 2015.

10. Tracking Ions from Tap Water to Ultrapure Water

This poster focuses on the removal of ions all along a water purification chain, from tap water to ultrapure water. Download this resource to learn about the efficacy of a water purification chain combining multiple technologies, such as reverse osmosis, electrodeionization and polishing on selected high purification media.

9. Ten Easy Steps for Cleaning a Spill in the Biosafety Cabinet

A spill within the biological safety cabinet can happen to even the most seasoned laboratory user. Taking precautionary measures before and during your work with hazardous materials could help keep you and others safe. Download this white paper for ten simple steps to keep you and your laboratory safe.

8. Eppendorf 96-Well Cell Culture Plate: A Simple Method for Minimizing the Edge Effect in Cell-Based Assays

Download this application note for a simple and convenient method to minimize evaporation using the Eppendorf 96-Well Cell Culture Plate. The plate offers two options for insulating the wells; a moat surrounding the edge wells and the complete chimney design of the inner wells allow filling of the complete inter-well space with liquid. This results in a more homogeneous distribution of temperature and humidity throughout the plate and reduces the edge effect.

7. Cell Cultivation without Contamination

This white paper compares different contamination control concepts for CO2 incubators from the user’s perspective. Read how BINDER CO2 incubators offer a conclusive concept for contamination avoidance, simplifying routine spray/wipe disinfection and enabling automatic auto-sterilization.

6. Improved Resolution for Paraquat and Diquat: Drinking Water Analysis Using the CORTECS UPLC HILIC Column

Diquat and paraquat are doubly charged quaternary ammonium herbicides, used extensively worldwide to control both crop and aquatic weeds. Both diquat and paraquat are too polar to be retained by reversed-phase liquid chromatography on C18 columns. Download this application note to learn how UPLC separation using a CORTECS UPLC HILIC Column significantly improved the retention and resolution of both analytes, allowing for detection down to 500 ppt by UV detection alone.

5. Improved Handling of Cells and Protection against Contamination

Although the T-flask is the most widely used vessel for routine maintenance of cell cultures, compared to dishes and plates, the T-flask provides only limited access to the cells due to its narrow neck which makes handling with pipettes and cell scrapers inconvenient. Download this note to learn how Eppendorf Cell Culture Flasks offer some unique benefits combining best protection against contamination with simplified and ergonomic handling.

4. Separation of Proteins and Peptides with Sartobind® Membrane Adsorbers

In this application note, ion exchange chromatography and Sartobind® membrane adsorbers were used to separate protein and peptide mixtures. All samples were prefiltered with a Minisart filter having 0.2 μm or a 0.45 μm pore size.

3. Infographic: How to Become a Pipetting Expert

Reverse pipetting, problem liquids, getting the angle right: Pipetting like a pro can be more complex than it seems. Download this Eppendorf infographic to stay on top of your most difficult pipetting tasks. These tips and tricks will help you to get consistent and reproducible results.

2. Best Practices in Handheld Pipetting

Download this application note to discover best practices in relation to handheld pipetting and maximizing accuracy of dispensing, including the optimization of pipetting performance.

And finally, the most downloaded application note of 2015 was….

1. Good Laboratory Practice - and How It Affects You

Good Laboratory Practice (GLP)-compliant testing in the laboratory can prevent pharmaceutical industry catastrophes, such as the thalidomide scandal in the 1970s, and 2012’s contaminated drugs meningitis outbreak in the USA. This quality assurance system encompasses all organizational structures, and the sequence of analyses and tests for authorization of a medicinal product. Download this white paper for a comprehensive overview of the fundamentals of GLP procedures.

Look out for the latest application notes and methods coming in 2016!

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Autoclaves and SterilizersAutoclaves and sterilizers are commonly used to sterilize laboratory equipment, instruments and growth media using constant pressure and temperature. When choosing an autoclave or sterilizer consider medical or laboratory application, size, loading and sterilization method. Sterilization methods include flame (Bunsen burners), heat (steam or dry), chemical and UV.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.IncubatorsLaboratory incubators are used to provide a stable environment to cultivate and store samples in, giving you optimal control of temperature, humidity, oxygen and CO<sub>2</sub> levels. Incubators are necessary equipment for cell culture and microbial research. Control temperature with heated or refrigerated incubators, or the levels of CO<sub>2</sub> and oxygen with CO<sub>2</sub> incubators. Using environmental chambers, create a humid climate for testing samples. Find the best incubators in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Pipettes and DispensersPipettes and dispensers are essential lab equipment used to dispense measured volumes of liquid accurately. Explore a range of manual and electronic pipettes, including single and multichannel, serological and volumetric pipettes, as well as a range of pipette tips. Bottle-top dispensers are also available for simple and fast solvent dispensing. Find the best pipettes & dispensers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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