Molecular Devices Introduces ScanLater Western Blot Detection System

15 Oct 2013
Sarah Thomas
Associate Editor

Product news

Molecular Devices announced today the launch of the ScanLater™ Western Blot Detection System. The first of its kind system combines western blot membrane detection with a multimode microplate reader platform, eliminating the need to acquire a separate dedicated western blot system, saving both lab space and budget. The system is compatible with Molecular Devices’ SpectraMax® i3 and SpectraMax Paradigm® Platforms.

ScanLater System is a time-resolved fluorescence (TRF)-based western blot detection assay, an optimal detection method that reduces stray excitation light, resulting in lower background noise and higher sensitivity. The ScanLater System comprises the ScanLater Western Blot Detection Cartridge, ScanLater Western Blot Kit and image acquisition software powered by SoftMax Pro® Software. ScanLater Western Blot Kits contain europium-labeled secondary antibodies designed to work with existing primary antibodies without further optimization. This substrate-free method of western blot detection not only outperforms traditional chemiluminescence and fluorescence-based western blot detection, it also allows membrane detection at any time.

Users can add western blot detection capability to the SpectraMax i3 or SpectraMax Paradigm Platform in just minutes by installing the ScanLater Western Blot Detection Cartridge. The cartridge is driven by SoftMax Pro Software, enabling users to run both microplate-based assays and western blot membrane detection from one software platform. SoftMax Pro Software provides a simple interface for acquiring .tif based images which may be printed, saved or exported to third party analysis packages. Using an add-on for ImageJ in SoftMax Pro Software, ScanLater System images are seamlessly exported with a single click.

Kevin Chance, President at Molecular Devices, commented: “Molecular Devices is committed to developing novel applications for our microplate customers, offering them the widest range of utility possible. The addition of the ScanLater Western Blot Detection System follows the introduction of the MiniMax Imaging Cytometer earlier this year, with the SpectraMax i3 System now leading the market in terms of application breadth.”

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Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Blotting
Molecular Devices Introduces ScanLater Western Blot Detection System