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Mobile phase optimization for oligonucleotide analytical methods by LC-MS

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Join us on Tuesday, June 16, to learn how to improve overall method robustness and sensitivity for oligonucleotide method development

2 Jun 2020
Georgina Wynne Hughes
Editorial Assistant

Expert insights

Brian Rivera,Global Product Manager for Bioseparations Products at Phenomenex
Brian Rivera, Global Product Manager for Bioseparations Products at Phenomenex

The use of alkylamine ion pairing reagents and perfluorinated alcohols in the analysis of oligonucleotides by LC-MS techniques has been the mainstay in characterization and quantitation methods for synthetic oligonucleotides for decades. Often, analytical chemists default to relatively high concentrations of both.

But what concentration of ion pairs do you need for optimal chromatographic performance? And, how much does perfluorinated alcohol concentration contribute to electrospray ionization efficiency?

In this webinar, Brian Rivera, Global Product Manager for Bioseparations Products at Phenomenex, explores modulation of both mobile phase additives, including different alkylamine ion pair options outside of the commonly used triethylamine.

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Key learning objectives:

  • Determine experimental parameters to ensure proper experimental design during method development
  • Understand the role of mobile phase and column chemistry in LC-MS methods for oligonucleotides
  • Learn how to improve overall method robustness and sensitivity for oligonucleotide method development

Who should attend?

  • Analytical method developers looking to improve separation and method robustness for oligonucleotides
  • Bioanalytical scientists needing to improve method sensitivity
  • Anyone new to oligonucleotide characterization by LC-MS

The live webinar takes place on Tuesday, June 16, 2020, at:

  • 16:00 BST
  • 17:00 CEST
  • 08:00 PDT
  • 11:00 EDT

Register to watch the full webinar here>>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.

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Mobile phase optimization for oligonucleotide analytical methods by LC-MS