Mo Bio to Host an Educational Seminar at ASM 2010

16 May 2010
Sarah Sarah
Marketing / Sales

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Visit MO BIO at booth 1020 to see the latest innovations in sample prep kits and equipment. Also, find out more about an educational seminar with information on DNA extraction from microbiological samples entitled: Yield, Quality and Purity: Considerations for DNA Extraction from Complex Microbiological Samples.

Yield, Quality and Purity: Considerations for DNA Extraction from Complex Microbiological Samples
Guest Speaker: Janet K. Jansson, PhD, Professor and Senior Staff Scientist Molecular Microbial Ecology Group, Earth Science Division, Lawrence Berkeley Laboratory
When: May 24th, 7:00pm
Where: Manchester Grand Hyatt, San Diego, CA
1 Market Place
San Diego, CA 92101

Problems achieving high yields and purity DNA are exaggerated in microbial samples, and especially those from complex environments (ex. soil, biofilms, gut) because of the variety of tough to lyse microorganisms and the high level of enzymatic inhibitors that co-purify with nucleic acids.

Successful microbial community analysis depends on high efficiency extraction and purification that accurately reflects the quantity of DNA or RNA in a sample. Environmental samples can have extreme differences between neighboring collection sites, collection depths, or even between individual gut microbiota, increasing the challenge of accurate profiling of different ecological habitats. This workshop will discuss considerations for obtaining high and accurate yields and purity of DNA from various samples isolated from environmental sources. The introduction will provide an overview of some popular misconceptions to watch for when analyzing DNA. Janet Jansson will present her work that focuses on the analysis of microbial ecology of diverse sample types and the importance of DNA yield, quality and purity in research.

Attention, space is limited! There are only 200 spots available. Make sure to register in advance to ensure your spot: simply send an email with your name and company name to info@mobio.com, with "ASM Seminar" as a subject line.

Visit booth 1020 to find out more.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.
Mo Bio to Host an Educational Seminar at ASM 2010