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Millipore Provides Labeled Secondary Antibodies

Merck
2 Apr 2007

Product news

Millipore Corporation today announced the availability of high-quality, labeled secondary antibodies used in single- and double-labeling experiments, fluorescence-activated cell sorting (FACS), enzyme-linked immunoadsorbent assay (ELISA), detection assays and Western blotting applications.

This series of antibodies is available either unconjugated or conjugated to an enzyme, biotin or fluorophore. All of Millipore’s labeled secondary antibodies are manufactured to exacting standards to provide high quality performance.

Millipore’s enzyme-labeled secondary antibodies, including the hyper-HRP labeled antibody, offer unparalleled sensitivity and permeance in immunohistochemistry applications. The enzyme conjugated antibodies, depending on application, may produce either a protein luminescence or color the proteins to be visible on the membrane.

Biotin conjugation provides high levels of signal amplification with minimal cost. Combining biotinylated secondaries with a matching line of conjugated streptavidin partners, signals can be multiplied quickly, enhancing their sensitivity in all applications.

Fluorescently labeled antibodies are available conjugated to a variety of flourophores spanning the entire visible and UV spectrum. The type of fluorochrome needed is determined by considering the individual application, available equipment and filters needed to measure fluorescent emission wavelengths.

Millipore’s Bioscience Division provides innovative tools, services and biological reagents that drive advancements in biomedical and academic research as well as support the discovery and development of new pharmaceuticals. The product portfolio has been further enhanced with the 2006 addition of Chemicon, Upstate and Linco product lines, allowing Millipore to provide the most complete offering of life science tools on the market. Our customers work in leading research laboratories across a variety of industries throughout the world. Millipore improves their laboratory productivity and efficiency through optimized workflows.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Millipore Provides Labeled Secondary Antibodies