Milliplex High Sensitivity Panel Introduced by Merck for Faster, More Economical Cytokine Assays

7 Dec 2016
Sophie Marsh
Administrator / Office Personnel

Product news

Smaller sample volumes and four times higher throughput than traditional assays offered

Merck, a leading science and technology company, has launched the MILLIPLEX® MAP Human High Sensitivity cytokine panel for faster and more cost-effective human cytokine assays. The new panel allows researchers to achieve four times higher throughput than traditional assays while using smaller sample sizes.

The new assay is the first 384-well kit for use with the Luminex FLEXMAP 3D® platform. Researchers can now simultaneously measure multiple protein biomarkers in a single sample, collecting up to 366 data points per plate in the same time it would take to run a 96-well plate. The new kit delivers high specificity and sensitivity and requires less than 10 µm of sample.

"The availability of validated 384-well kits is an important new tool for customers with limited sample volumes who require high throughput, specificity and selectivity," said Jason Apter, Head of Research Solutions Strategic Marketing & Innovation, Life Science. "Simply put, researchers with limited sample volumes will get more results, faster."

Rush Medical College's laboratory has been using customized designed 384-well assays on the Luminex FLEXMAP 3D® system for nearly two years.

"Now that Merck is offering off-the-shelf assay kits, we expect to run even more assays in this format, which will help expedite our research," said Jeffery Borgia, Assistant Professor, Rush Medical College.

Assays help researchers identify and quantify soluble proteins and peptides that play an important role in the immune system and its response to antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease, sepsis and cancer.

The new MILLIPLEX® MAP Human High Sensitivity cytokine panels expand Merck's existing portfolio of immunoassays for human cytokines, essential tools for academic centers, contract research organizations and biotech/pharma companies.

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High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CytokinesCytokines are signaling proteins critical for cell communication in the immune system. They regulate inflammation, immune responses, and cell growth. Cytokine research is vital for understanding disease mechanisms and developing therapies. Explore tools for cytokine detection and analysis in our directory.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.