SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Merck Millipore Launches Kit for Analyzing NFкB Translocation by Imaging Flow Cytometry

Merck KGaA, Darmstadt, Germany
19 Nov 2014

Product news

Merck Millipore, the Life Science division of Merck, today introduced the Amnis® NFкB Translocation kit, which allows researchers to better study the nuclear translocation of NFкB, a transcription factor that plays a central role in regulating key mammalian cell processes, including proliferation, inflammation, immune and stress responses. The new kit uses imaging flow cytometry to obtain statistically significant quantitative assessment of NFкB translocation, as well as visual identification of the translocation at a single-cell level.

The optimized kit, which works with cultured cell lines and whole blood cells, conveniently contains directly-conjugated anti-Human NFкB monoclonal antibody, 7-AAD dye and required buffers. Using the dedicated Nuclear Localization Wizard in the IDEAS software, NFкB translocation can then be studied and quantified in an objective, statistically robust manner. The kit is designed for use with the Amnis® ImageStream®X Mark II and the Amnis® FlowSight® imaging flow cytometers, systems which combine the quantitative power of flow cytometry with the spatial information provided by microscopy.

"With a central role in many cellular responses and human diseases, NFкB continues to be an attractive target for drug discovery," said Patrick Schneider, Ph.D., Head of Bioscience. "Availability of the Amnis® imaging flow systems in combination with the new NFкB Translocation kit provides immunology and cell biology researchers an easier, higher throughput way to further explore the mechanisms of action of this important transcription factor and reveal insights into targeting its signaling pathway for therapeutic purposes."

Related products

Request Quote for All Products

Amnis® NFkB Translocation Kit

Merck KGaA, Darmstadt, Germany

The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor 488, the nuclear stain 7-AAD along with the fixation solution, assay buffer and permeabilization buffer to rapidly detect and quantitate NFkB's translocation from the cytoplasm to the nucleus. Nuclear Factor kappa B (NFκB) transcription factor plays a central role in regulating many key processes in mammalian cells, including differentiation, cell growth, inflammation, immunity, and stress responses. The p50/p65 heterodimer is considered to be the most abundant form of NFκB. NFκB is held in an inactive state in the cytoplasm by its inhibitor IkB. Phosphorylation of serine residues on the IkB proteins marks them for degradation via ubiquitination, therefore allowing activation of the NFκB complex. Activated NFκB complex translocates into the nucleus. The Anti-Hu NFκB Alexa Fluor® 488 antibody recognizes NFκB p50 which is a component of the NFκB p50/p65 heterodimer.The Amnis NFkB translocation kit includes the conjugated antibody Anti-Hu NFkB (p50) Alexa Fluor® 488, the nuclear stain 7-AAD alonge with the fixation solution, assay buffer and permeabilization buffer. This 2 color imaging flow cytometry kit along with the Amnis imaging cytometry platforms are designed to detect and quantitate NFkB translocation from the cytoplasm to the nucleus. Applications: Flow Cytometry

(1)

Links

Merck KGaA, Darmstadt, GermanyCompany website

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.CytometryCytometry is a broad term for the quantitative analysis of cells and cell systems. The most popular methods to study cytometry are flow cytometry and image cytometry.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.

Related articles