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Maximal recovery of DNA and RNA from tiny samples

QIAGEN
2 Oct 2007

Product news

Two new kits from QIAGEN provide maximal nucleic acid yields from very small cell and tissue samples: the RNeasy® Plus Micro Kit, which purifies RNA, and the AllPrep® DNA/RNA Micro Kit, which simultaneously purifies DNA and RNA. Both kits are specially designed for tiny samples such as fine-needle aspirates and laser-microdissected cryosections.

The RNeasy Plus Micro Kit achieves optimal sample lysis with Buffer RLT Plus to maximize RNA yields. Genomic DNA is effectively and rapidly removed in a single centrifugation step using unique gDNA Eliminator spin columns. High-quality total RNA is then purified using RNeasy MinElute® spin columns, which allow an elution volume of as low as 10 µl.

The AllPrep DNA/RNA Micro Kit enables simultaneous purification of DNA and RNA from the same sample, avoiding the need to split the sample into two for separate purification procedures. The lysate is first applied to an AllPrep DNA spin column to isolate genomic DNA, and then to an RNeasy MinElute spin column to isolate total RNA.

Total RNA purified with both kits is well suited for any application, such as RT-PCR, real-time RT-PCR, and microarray analysis. Genomic DNA purified with the AllPrep DNA/RNA Micro Kit is suitable for both qualitative and quantitative analyses, including PCR, real-time PCR, genotyping, and comparative genome hybridization (CGH).

The RNeasy Plus Micro Kit and AllPrep DNA/RNA Micro Kit are intended for research use. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

Trademarks: AllPrep®, MinElute®, RNeasy® (QIAGEN Group).

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Maximal recovery of DNA and RNA from tiny samples