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Malvern Scientists will Lead Protein Characterization Workshop at PEGS Summit

Malvern Panalytical
8 May 2011
bridget bridget
Laboratory Director

Product news

Drs Kevin Mattison*, Ulf Nobbmann and Jason Sanchez of Malvern Instruments will lead a workshop on ‘Characterization techniques for protein therapeutics’ - at the 7th Annual PEGS - Protein & Antibody Engineering Summit being held in Boston MA, from 9-13 May 2011. As a corporate sponsor, Malvern Instruments will also be exhibiting and company experts will be on hand to discuss light scattering, size exclusion chromatography (SEC) and dynamic imaging technologies.

The workshop will cover the theory and general application of both traditional and emerging technologies suitable for protein therapeutic characterization, across the applicable size range of 1 nm to 100 µm. Participants will seek to answer questions such as: What’s the difference between Tm measured by DSC, CD, & DLS? Can DLS be used to confirm SEC results? What’s the relevance of protein charge on formulation stability? Can particle counting be achieved in the 100 nm to 1 µm size range? What role does protein aggregate “morphology” play?

Malvern solutions for characterizing proteins and other macromolecules include: the Zetasizer µV with OmniFACE and OmniSEC, a new SEC-LS detector package dedicated to protein analysis; Zetasizer Nano systems, which use dynamic and static light scattering to measure size and molecular weight; Viscotek size exclusion chromatography systems/detectors for molecular weight and molecular structure characterization; Sysmex FPIA-3000 for dynamic imaging, size and shape characterization of protein aggregates.

For details of Malvern’s protein solutions visit the company article page.

*Kevin Mattison is Principal Scientist, Bioanalytics; Ulf Nobbmann is Senior Applications Specialist, Nanometrics; and Jason Sanchez is Product Manager GPC/SEC Technologies, all at Malvern Instruments.

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Malvern PanalyticalCompany website

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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.

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Malvern Scientists will Lead Protein Characterization Workshop at PEGS Summit