Make significant cost savings in concentrating proteins...

29 Mar 2006
Kerry Parker
CEO

Product news

Genevac, leader in solvent removal technology, reports on the significant cost savings routinely achievable using their popular miVac centrifugal evaporator for concentrating proteins compared to traditional membrane concentration methods.

For laboratories looking to pre-concentrate protein solutions prior to crystallography, size exclusion chromatography or electrophoretic techniques - membrane centrifugation has traditionally been the method of choice. However, for laboratories that use large numbers of membranes per year there is an alternative, the miVac. There is a one off cost that will pay back in months, and greatly reduce spending on consumables. Additionally, unlike the traditional technique where some sample is often lost due to protein binding to the membrane, the centrifugal evaporator sample concentration technique ensures complete sample recovery even when taking proteins to very high concentrations.

The miVac range has been designed to meet the requirements for careful and speedy drying in most biological applications. Special methods for drying aqueous and alcohol-based samples are included in all the miVac models. A range of concentrators feature capacity from six to twenty stacked shallow-well microplates or 48 to 200 micro centrifuge tubes of 1.5ml size. The miVac concentrators feature full LCD display with digital controls and programming of time and temperature. A range of suitable vacuum pumps is also available, including 2-head and 4-head diaphragm pumps and an ultra-low vacuum scroll type pump.

To request further information on how you can make significant savings in concentrating proteins using a miVac centrifugal evaporator please contact Genevac on telephone +44-1473-240000 or email salesinfo@genevac.co.uk

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.CentrifugesCentrifuges are used to separate particulates suspended in a liquid via the application of a centrifugal force including cells, macromolecules, nanoparticles and precipitates. Centrifuges come in three sizes: microcentrifuges, benchtop and floor-standing centrifuges, depending on the volume and number of samples that require separation. When choosing a centrifuge, consider its maximum separation power indicated by its relative centrifugal force (RCF) value. Low force centrifuges reach a maximum RCF of 8,000 g, high-force centrifuges can reach maximum RCF values of 80,000 g. For extremely high forces, consider ultracentrifuges (up to 800,000 g). Refrigerated and vacuum centrifuges also known as concentrators are available for more specialized applications. A range of volumes are available for centrifuge tubes and microfuge tubes, depending on sample sizes. Find the best centrifuges in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.Evaporators and Freeze DryersCentrifugal evaporation is used for solvent removal from a sample. This is a useful technique for sample concentration or sample drying. Many centrifugal evaporators are modular with a central vacuum pump for generating low pressure. Useful features of evaporators or vacuum ovens include minimal solvent bumping, solvent resistance, computer control, temperature regulation and benchtop design.
Make significant cost savings in concentrating proteins...