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Magnetic Protein Isolation—Based on MACS® Technology at HUPO 2009

Miltenyi Biotec B.V. & Co. KG
20 Sept 2009
Sarah Sarah
Marketing / Sales

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Visit Miltenyi Biotec at booth 17 to find out more about magnetic protein isolation. At the heart of MACS® Technology are nanometer-sized, non-sedimenting, and superparamagnetic MicroBeads. Coated with monoclonal antibodies or other proven binding moities, such as streptavidin, or Protein A and G, the MicroBeads specifically bind their target proteins—while minimizing non-specific binding.

The magnetically labeled sample can be easily separated in a MACS Column placed in the strong magnetic field of a MACS Separator. Thus, time-consuming centrifugation and tedious pre-clearing steps become unnecessary. All binding, washing, and elution steps are performed in a single column.

Besides single-sample isolations, the parallel processing of up to 96 samples can be performed in a fully-automated approach.

Products are available for the isolation of c-myc-, GFP-, GST-, HA-, and His-tagged proteins and for immunoprecipitation (IP) and Co-IP of virtually any protein. Besides tools for isolating biotinylated proteins and their interaction partners, a unique kit is offered for the isolation of native transcription factors.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.

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Magnetic Protein Isolation—Based on MACS® Technology at HUPO 2009