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Magnetic Beads Developed for Proteomic Applications

AMSBIO
28 Nov 2011

Product news

AMSBIO is pleased to announce the availability of MagSi-proteomics beads - magnetic beads designed for the purification, concentration and desalting of peptides and protein digests. The surface of the beads has been modified with C4, C8 and C18-alkyl groups optimised for reversed phase applications.

Sample purity and throughput are areas of key importance for proteomics researchers. Magnetic beads offer a convenient solid support for a variety of assays and procedures based on affinity purification. They are especially well suited for automated procedures because instrumentation is available to easily mix, incubate and separate the magnetic beads in 96-well plates without columns or centrifugation. This ease-of-use enables rapid processing of many samples and seemless integration into automated workflows.

MagSi proteomics beads have been demonstrated as a powerful tool in desalting of proteomics samples after protein digestion and prior to mass spectrometry. Offering higher throughput and cost efficiency compared to tip-based separations - MagSi proteomics beads efficiently bind and elute even tiny sample fractions. Peptide amounts as low as 20 - 50 ng can be desalted by MagSi proteomics C8 and C18 beads and analyzed by state of the art MALDI-TOF instruments. Typically for small peptides MagSi-proteomics C8 or C18 beads are suited best, whereas MagSi proteomics C4 and the MagSi-WCX are optimised for larger proteins.

The high magnetic strength of MagSi beads makes them applicable for both, manual and automated/robotic fractionation, because the beads will typically collect in less than 1 minute when magnetic force is applied. This quick and complete separation gives very good reproducibility since no beads will be lost during washing steps. Furthermore the quick protein adsorption, desorption and magnetic collection typically shortens significantly the protocol time over conventional column based ion exchange chromatography.

For further information please visit the company article page.

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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.Biopharmaceutical AdvancesBiopharmaceutical advances follow the development of pharmaceuticals derived from biotechnology, also known as biotechnology medicines. Biopharmaceuticals may be produced from cell lines, plants, or microbial cells. Important considerations of biopharmaceutical use include application, cost, production process and purification.Magnetic Beads

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Magnetic Beads Developed for Proteomic Applications