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Lullaby® - siRNA transfection reagent: a sweet song for gene silencing

OZ Biosciences
18 Sept 2006
Olivier Zelphati
Chief Scientific Officer

Product news

OZ Biosciences is pleased to announce the launching of a new lipid-based transfection reagent for siRNA experiments, highly efficient at low doses of siRNA and avoiding non-specific effects.

Lullaby® - siRNA transfection reagent is the ideal reagent dedicated to the transfection of siRNA. Based on an innovative technology, this new lipid-based reagent achieves extremely efficient siRNA delivery into cells. Lullaby® - siRNA transfection reagent protects siRNA from extracellular degradation and has an outstanding capacity to destabilize cells membrane, allowing delivery of important siRNA amounts into the cytosol.

Transfection is very efficient and highly reproducible, even with low doses of siRNA. Lullaby® allows high gene silencing levels in various conditions: cells, concentrations, targets, culture conditions, etc. It has been clearly shown that high concentrations of siRNA can cause off-target effects. Thus, 1 to 20nM siRNA concentrations are ideal for transfection. Lullaby® - siRNA transfection reagent is efficient starting from 0.1nM siRNA and optimal at 5 to 10nM, avoiding non specific effects.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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Lullaby® - siRNA transfection reagent: a sweet song for gene silencing