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Lonza to Highlight a New 3D Culture System Reforming Spheroid Culture and Analysis at AACR 2016
5 Apr 2016Alex Waite
Editorial Assistant
Industry news
Lonza will demonstrate the RAFT™ 3D Culture System at AACR 2016 and highlight new applications and optimized protocols that allow researchers to overcome some of the challenges previously associated with working with 3D cultures.
RAFT™ 3D Cell Culture System uses a high-density collagen scaffold to closely mimic the extracellular matrix of native tissues. Cancer cells can be embedded in the collagen matrix, and many of them form tumor-like structures.
Delegates visiting the Lonza booth #1930 can discover:
- How the translucent properties of RAFT 3D Culture System enable visualization of tumor-like structures with subcellular resolution under a fluorescence microscope
- How modification of a standard 2D analysis method enables use of Lonza’s ViaLight™ Assays to assess cell viability in 3D cell culture.
- How the RAFT™ System permits different cancer cell types to show distinct morphologies, as well as characteristic tumor migration and invasion patterns. A recent study demonstrated how this can help researchers understand how to maximize the therapeutic potential of drugs in a more “in vivo”-like cell culture system.
- How to overcome the challenge of transfecting cells in 3D cell culture by using Lonza’s Nucleofector™ Transfection Technology to achieve high transfection efficiencies and good cell viability prior to transferring cells into 3D cell culture. This efficient approach creates spheroids that maintain transfected substrate for several days in culture
In addition, Lonza will present a poster in the tumor biology category titled “Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay” on Sunday, 17 April 2016, 1:00 pm to 5:00 pm, at the Convention Center, Halls G-J, Poster Section 28.
A further poster featuring the RAFT™ 3D Cell Culture System will be presented by Tarig Magdelin, Postdoctoral Research Associate at the Cancer Nanotechnology Group, University College London. “Engineering a Vascularized 3D "In Vitro" Model of Cancer Progression” will be presented on Tuesday, 19 April 2016, 1:00 pm to 5:00 pm at Poster Section 10.
More information about these new applications of RAFT™ 3D Cell Culture System for cancer research will be available at Lonza’s booth #1930 at AACR 2016.
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ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay Kit
Lonza Group LtdViaLight® Plus BioAssay Kits are designed to provide unprecedented speed and sensitivity for cytotoxicity and cell proliferation studies. The ViaLight® Assay incorporates bioluminescent detection of cellular ATP as a measure of viability. Measurement of ATP is the most accurate, effective, and direct way of determining the number of living cells in culture. The ViaLight® Plus BioAssay is designed to deliver a high, stable luminescent signals for an extended period of time for greater experimental design flexibility. The easy two-step, non-radioactive assay is scalable for high-throughput applications in both 96-well and 384-well formats. The assay can be run on a variety of luminometers or scintillation counters.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.In Vivo Imaging Systems<i>In vivo</i> imaging systems, including pre-clinical imaging systems and medical imaging systems are used to non-invasively visualize and capture images of live animals and plants. Monitor the natural processes or diseases of your subjects using small-animal pre-clinical imaging systems, including single photon positron emission tomography (SPECT), positron emission tomography (PET), computed tomography (micro-CT), magnetic resonance imaging (MRI), X-ray radiography, ultrasound, fluorescence and bioluminescence imagers. Multimodal systems and software solutions are also available for correlative analysis of organ, tissue, cell, or molecular-level processes. Find the best in vivo imaging products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cancer CellsCancer cells are abnormal cells that divide uncontrollably, leading to the formation of tumors and the spread of cancer. Studying cancer cells is crucial for developing new treatments and understanding tumor biology. Explore cancer cell research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.SpheroidsSpheroids are clusters of cells that have been grown in 3D culture to be used as <i>in vitro</i> model systems. These 3D microtissues can be used for toxicology testing, DMPK studies and many other applications involving cell analysis.TherapeuticsTherapeutics are treatments designed to alleviate or cure diseases. These include pharmaceuticals, biologics, and gene therapies, which work by targeting specific disease mechanisms. Advances in personalized medicine and biologics offer new hope for patients with conditions that were previously difficult to treat. Browse our peer-reviewed product directory to find therapeutic solutions for various diseases; compare products, check user reviews, and get pricing directly from manufacturers.TumorsTumor research focuses on understanding abnormal cell growth that leads to cancer. Identifying biomarkers, studying tumor microenvironments, and developing targeted therapies are critical for advancing cancer treatment. Early detection and personalized treatment options are key to improving outcomes for patients. Browse our peer-reviewed product directory to explore tools for tumor research, diagnostics, and cancer therapies; compare products, read customer reviews, and get pricing directly from manufacturers.AACRThe American Association for Cancer Research (AACR) is a leading organization focused on cancer research and treatment. It provides a platform for scientists, healthcare professionals, and industry leaders to share knowledge and collaborate in the fight against cancer.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.