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Latest advances in protein biology

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From ultrafiltration and extraction to glycosylation and imaging, discover the latest technologies, top tips, and best practices for protein biology research

2 Nov 2020
Carrie Haslam
Associate Editor

Editorial article

Protein Biology SelectScience special feature 2020

In this new special feature, we pull together an array of top resources to help you stay up to date with the latest technologies and techniques for protein biology research. Learn how to improve your protein extraction yields; discover a method for highly sensitive gel imaging with reduced exposure to toxic materials; find out how glycosylation analysis is being used to inform recombinant protein selection for COVID-19 research; explore top tips and tricks to optimize your sample preparation workflows, and much more!

Application note - SelectScience Protein Biology special feature 2020

Lab ultrafiltration: Tips & tricks to optimize workflows

Discover how Vivaspin® Centrifugal Concentrators can help you effectively perform sample concentration and buffer exchange and learn top tips to improve recovery and speed up your workflows with Sartorius.

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Application note - Protein Biology Special Feature 2020

Gel imaging: Reduced toxicity and enhanced sensitivity

Learn how to achieve repeatable and precise imaging analysis with reduced exposure to toxic materials with the UVP GelStudio from Analytik Jena, offering high sensitivity and stability, with enhanced safety.

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Application note: Protein Biology Special Feature 2020

Improved extraction: Best practices for protein sample preparation

Download this guide from Bertin Instruments to learn how to achieve improved protein extraction yields from a variety of sample types. Here, two specific protocols using the Precellys Evolution are outlined.

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Application note: Bio-Techne Protein Biology Special Feature 2020

Spike protein: Selecting Spike RBD proteins for COVID-19 research

Learn how Bio-Techne uses direct fluorescent glycan labeling (DFGL) to analyze glycosylation of SARS-CoV-2 Spike proteins to help provide guidance on choosing the most suitable recombinant proteins for COVID-19 research.

Download Method

Interviews with leading scientists:

  • Co-directors of the ELRIG Research and Innovation 2020 meeting, Dr. Darren Cawkill and Dr. Jon Hutchinson, discuss current trends and challenges in protein biology and drug discovery. Read article>>
  • Prof. Christian Griesinger discusses the advantages of using ultra-high field nuclear magnetic resonance spectroscopy to investigate the structure of intrinsically disordered proteins (IDPs). Read article>>
  • Prof. Jane Dyson discusses the importance of IDPs for protein function and emphasizes the benefits of using nuclear magnetic resonance (NMR) for studying these protein mechanisms. Watch interview>>
  • Prof. Steve Hill describes his research on the ligand binding of cell surface GPCR and RTK proteins. Watch interview>>
  • Dr. Mark Soave highlights how Promega’s NanoLuc® Binary Technology split luciferase assay allows his team to examine protein:protein interactions of G protein-coupled receptors (GPCR) and other proteins in response to drug treatments. Watch interview>>
  • Dr. Richard Kay outlines how he uses mass spectrometry to measure gut hormone peptides related to diabetes, the challenges of detecting low-abundant peptides such as insulin and the benefits of performing peptide mass spectrometry over immunoassays. Watch interview>>

Related resources:

  • Meet the gel imaging system designed to improve workflow efficiency and fit just about anywhere. Read article>>
  • The future of drug discovery: Discover how scientists at Medicine Discovery Catapult are using biomarkers to tailor approaches to patient care, in this exclusive interview with Dr. Sally Price. Read article>>
  • For thermo-sensitive molecules such as proteins, sample preparation is a critical step before proceeding to molecular downstream analysis. In this application note, Bertin Instruments highlights the key considerations in protein sample preparation. Download method>>
  • Xing Wang, from Array Bridge, introduces a new technology for protein conformational analysis and explains how it is being used to study immunogenicity correlation. Watch presentation>>

Your recommendations:

Find out what researchers around the world are saying about the products they use! Eric Bankaitis, a research scientist at Powered Research, LLC., shared his opinion of the Precellys Evolution tissue homogenizer by Bertin Instruments.

For more of the latest news, straight to your inbox, become a member of SelectScience for free today>>

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Vivaspin® Turbo

Sartorius Group

Classical concentration and buffer exchange methods are labor intensive, generate excessive waste and suffer from sample loss and long process times. It can also be difficult to retrieve retentate samples from centrifugal ultrafilters when using standard pipette tips. To overcome these challenges, Sartorius has developed Vivaspin® Turbo - the ultimate range of centrifugal ultrafilters. These feature highly optimized designs and membrane options to offer the highest processing speeds, maximum biomolecule recoveries, and straightforward retentate retrieval.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel ImagingGel imaging captures high-resolution images of electrophoresis gels to analyze DNA, RNA, and proteins. Essential in molecular biology, this technique helps visualize results for accurate quantification and documentation. Discover top imaging systems and compare user-reviewed options in our directory.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Recombinant ProteinsProtein PurificationProtein BiologyProtein Quantification

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