JEOL Introduces a New Correlative Microscopy Tool for Observing Biological Samples and Materials in Atmosphere

JEOL introduces ClairScopetm, a first-of-its kind correlative microscopy tool that combines a high-end Light Microscope (LM) with a high-resolution Atmospheric Scanning Electron Microscope (ASEM). This enables uncompromised observation of samples in their native state using both LM and ASEM, significantly reducing sample preparation time and allowing dynamic observation of real time processes. The atmospheric SEM allows high resolution observation of wet biological samples such as cultured cells and imaging of materials samples in liquids.

High Resolution Observation of Life Science and Materials Experiments As They Occur

The ClairScope’s unique specimen dish makes it possible to add reagents, drugs, and other substances to the sample in order to perform experiments and observe reactions in both liquid and gas environments. For the first time, life science researchers can harness the powerful imaging capability of the SEM to observe biological processes such as platelet generation, distribution of sugar chains, and microbe growth. Materials scientists will be able to observe and record crystallization, electrochemical reactions, emulsion technology, self-assemblies, and dendrite growth as they occur.

Integrating LM and SEM with Control of Sample Environment

Researchers can control the specimen’s environment and stop processes at specific points in the experiment using the ClairScope to observe samples at atmospheric pressure in liquid or gas. The wide-field light microscope with emersion lens is co-axially aligned with the inverted electron column making it easy to switch between the light microscope and the electron microscope without repositioning the specimen dish. The specimen dish features an ultrathin SiN film window that allows transmission of the electron beam for SEM imaging while the sample is open to atmospheric pressure.

“The ClairScope features a high quality wide-field microscope integrated with the ASEM. When we combine SEM and LM, there is no compromise in working distance and there are no issues with vacuum in the ClairScope,” said Mitsuo Suga, ClairScope Project Leader.

Atmospheric SEM vs. Environmental SEM

The development of an Atmospheric SEM offers greater opportunities to observe samples in their native state than previously possible. “The advantage of this ASEM over an ESEM is that we can look at samples at room temperature and at atmosphere and we can watch those processes dynamically,” said JEOL USA Product Manager Donna Guarrera. “No lengthy drying processes, protective coating, or freezing of wet samples such as cultured cells, is required.”

The new ClairScope is being introduced at the Society for Neuroscience meeting in Chicago this week, where more than 30,000 scientists are involved in cutting edge research.