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Invitrogen Launches Seven-Minute Protein transfer Instrument

Invitrogen Limited
26 Oct 2006

Product news

Invitrogen Corporation, a leader in life science research, announces the launch of its new iBlot Dry Blotting System. This benchtop instrument is designed to reliably transfer proteins from polyacrylamide gels to nitrocellulose membranes in preparation for downstream analysis in just seven minutes.

We have been using the iBlot System for over a year now as an alpha test site, and during that time we have been able to assay hundreds of HTS hits in a variety of assays, said Jeff Szwaya, scientist at ArQule, Inc. (Woburn, MA). The extremely short transfer time produces clean, sharp bands and has yielded timely and reliable results with a markedly increased throughput."

Traditional wet or semi-dry systems for transferring proteins from gels to membranes rely on complex buffering systems, layers of filter paper, and can take several hours. The iBlot Dry Blotting System uses two solid-phase ion reservoir matrixes in pre-assembled transfer stacks to blot proteins in seven minutes or less. The proprietary formulation of the transfer stacks and the integrated nitrocellulose membrane ensure reproducible protein transfers every time. The self-contained iBlot System does not require additional buffers or an external power supply, enabling western analysis to be completed in a single work day, rather than 24 hours or more as with traditional systems. Complemented by other products such as NuPAGE Novex pre-cast polyacrylamide gels, Zymed and BioSource antibodies, and Molecular Probes fluorescent probes, the iBlot System enhances Invitrogens comprehensive solutions for western blotting and protein analysis.

By introducing the first dry blotting system to the market, Invitrogen is leading innovation in protein electrophoresis and western blotting, noted Cory Szafranski, marketing manager of protein analysis at Invitrogen. The iBlot Dry Blotting System is especially ideal for quantitative work because it can quickly and uniformly transfer proteins of both large and small molecular weights, and the system has been rapidly embraced by our customers.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Invitrogen Launches Seven-Minute Protein transfer Instrument