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Introducing the VeriFi library amplification mix

PCR Biosystems

Advanced library amplification to reduce GC-bias and push the limits of NGS data

10 Apr 2023
James Li
Blood Banking Scientist

Product news

PCR Biosystems has launched a new proofreading polymerase mix for NGS library preparation workflows. VeriFi™ Library Amplification Mix combines the powerful and robust performance of VeriFi™ Hot Start Polymerase with greatly reduced GC-dependent bias to push the limits of NGS data.

NGS enables researchers to quickly sequence entire genomes and transcriptomes of organisms. This technology unlocks insights into complex biological processes, ultimately enabling the development of targeted treatments and more effective vaccines and contributing to a better understanding of the natural world. Library amplification is a critical step of PCR-based NGS workflows. However, PCR is susceptible to GC bias, which compromises the quality of sequencing libraries and in turn negatively impacts resulting data quality. Researchers, therefore, typically look to reduce GC bias during NGS library amplification, so they can be confident in the insights they elucidate from the data generated.

VeriFi Library Amplification Mix has been specially engineered to reduce GC-bias. Incorporating the proven VeriFi Hot Start Polymerase with AptaLock hot start technology, the mix also utilizes proprietary chemistry to minimise GC-bias and enable reliable amplification for sequencing workflows. Having undergone testing on Illumina® platforms, PCR Biosystem reports that the mix was shown to have minimal bias, even during amplification at extremes of GC content ranging from 30.

“Empowering researchers to advance their research and unlock new insights is the driving force behind everything we do at PCR Biosystems,” stated Alex Wilson, Co-founder and Business Development Manager, PCR Biosystems. “And we are proud that we have developed this cutting-edge mix, which is cost-effective and will truly benefit researchers, giving them more and better data when undertaking costly and laborious NGS experiments.”

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.NGSGC-MSGas Chromatography-Mass Spectrometry (GC-MS) combines the separation power of gas chromatography with the detection capabilities of mass spectrometry, allowing for the analysis of complex mixtures. It is widely used in environmental, forensic, and pharmaceutical analysis. Explore GC-MS systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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