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Introducing an Affordable Capillary Gel Electrophoresis Analyzer for DNA

Precision Biosystems
9 Feb 2015
Sarah Thomas
Associate Editor

Product news

The Qsep100 DNA Analyzer from Precision Biosystems performs rapid, high sensitivity assays at lower cost per sample compared to similar systems.

Capillary gel electrophoresis technology is recognized as an optimal approach to performing post PCR separation, detection of DNA and RNA fragments and assessing the quality of genomic DNA. The Qsep100 DNA Analyzer, by Precision Biosystems, uses micro-capillary gel electrophoresis combined with fluorescence detection, and is capable of performing 200 assays automatically as quickly as 2 minutes per sample. With costs per sample as low as $0.10 each, Qsep100 is a fast and highly sensitive analyzer for DNA and RNA assays.

“There is no question, capillary gel electrophoresis with LED-induced fluorescence is a powerful analytical tool for DNA analysis and immunoassays, effectively replacing time-consuming and labor intensive slab-gel electrophoresis technology,” says Russ Yukhananov, CEO of Precision Biosystems. “Data posted on our website shows our Qsep100 system exhibited excellent reproducibility in every experiment we performed,” he added.

The Qsep100 DNA Analyzer quickly resolves DNA fragments in the range of 10 to 20,000 base pairs (bp) with 2 to 4 bp resolution. The design features a disposable pen shaped cartridge filled with gel that incorporates a short fused silica capillary. The sample is automatically injected into the cartridge where it migrates through the capillary, and fragments are excited by a super bright light emitting diode (LED) and signal detected by photomultiplier. A radio frequency identification (RFID) chip built into the pen-shaped gel cartridge tracks the number of runs, the type of gel cartridge used, and the sample number. The compact analyzer is supplied with a 12-well or 96-well sample loader. System set up requires an external nitrogen gas source or air pump and standard electrical supply. Scientists capture and manage experimental data using the supplied software with its intuitive interface, and data transfer is facilitated with a USB connection in spreadsheet compatible format.

The Qsep DNA Analyzer is available for onsite demonstration. For more information click on the ‘request info’ button below.

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Capillary ElectrophoresisCapillary electrophoresis (CE) is used to separate molecules based on charge, size and hydrophobicity. Pressure, voltage or a vacuum is used to introduce the sample to fused silica capillaries. Fluorescence, UV/Vis, UV or diode array detectors are used to visualize components. Types of CE systems include capillary electrochromatography (CEC), capillary zone electrophoresis and capillary gel electrophoresis. Find the best capillary electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.

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