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Integrated DNA Technologies to Present on Rapid and Reliable Method of Gene Cloning at Antibody Engineering and Therapeutics

Integrated DNA Technologies Inc.
2 Dec 2013
Sarah Thomas
Associate Editor

Product news

Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, will be speaking at the upcoming Antibody Engineering and Therapeutics conference in Huntington Beach, CA, December 9-12. Michel Cannieux, PhD, MBA, Director of Product Commercialization, Synthetic Biology at IDT will discuss a novel, rapid and reliable method to build and clone genes of interest at a fraction of the cost of full gene synthesis.

The talk, entitled “Rapid and Reliable Gene Construction with gBlocks™ Gene Fragments and Gibson Assembly™ Method” will take place at 12.00 on December 11. As double stranded, sequence verified DNA blocks up to 750 base pairs, gBlocks Gene Fragments have high sequence fidelity. As a result, they are ideal for use in a broad range of biological applications. This presentation will review the assembly of gene fragments using the Gibson Assembly Method and discuss design, protocols and applications.

Michel Cannieux holds a PhD in Plant Molecular Biology and has over 15 years’ experience in the life science industry, developing and supporting tools and solutions for functional analysis, including RNAi and synthetic biology.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Therapeutic AntibodiesTherapeutic antibodies are antibodies engineered for use in treating diseases. They can be designed to target specific antigens, blocking harmful interactions or modulating immune responses. Therapeutic antibodies are critical in treating conditions like cancer, autoimmune disorders, and infectious diseases. Explore the best therapeutic antibody products in our peer-reviewed product directory; compare options, check reviews, and get pricing directly from manufacturers.RNAi

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