SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Integrated DNA technologies launches complete NGS library preparation solution for monkeypox

Integrated DNA Technologies Inc.

Leading genomics solutions provider debuts complete NGS library preparation to support the identification of novel monkeypox variants in response to growing researcher demands for viral surveillance solutions

31 Aug 2022
Dora Wells
Clinical Content Editor

Industry news

With the World Health Organization declaring the monkeypox virus (MPXV) a Public Health Emergency of International Concern, and more than 47,000 total cases across 99 countries reported to date and growing, Integrated DNA Technologies (IDT) is supporting researchers conducting monkeypox surveillance with a new next generation sequencing (NGS) solution. Designed as part of IDT’s NGS Tech Access, the program is intended to accelerate innovation by enabling access to the most advanced research tools that are still in development.

The xGen™ Monkeypox Virus Amplicon Panel is a complete NGS library preparation solution that supports the genotyping of MPXV within a sample. Leveraging IDT’s well-established NGS amplicon technology, the single-tube workflow creates overlapping amplicons, goes from sample to sequencer in 2.5 hours with up to 1,536 unique dual indexing (UDIs) for multiplexed sequencing, and helps pinpoint evolving epidemiological patterns.

“With monkeypox cases continuing to rise, IDT responded with a new solution to support global efforts across the scientific community—because more discoveries can lead to better prevention and mitigation strategies,” said Steven Henck, vice president of R&D. “At IDT, we focus on using our team’s ability to deliver the right tools when they are needed because we believe that supporting and advancing the global research community benefits us all. The addition of the NGS monkeypox amplicon panel reinforces our mission to accelerate the pace of genomics and we’re proud to help researchers reliably identify and track the evolution of monkeypox.”

NGS surveillance powered by super amplicons

The infectious disease monitoring and surveillance field continues to face unprecedented pressure for accuracy and increased speed. IDT’s xGen Monkeypox Virus Amplicon Panel is a single-tube workflow that generates contiguous tiling of overlapping amplicons. Super amplicons enable novel variant discovery by maintaining genomic coverage even if a novel variant occurs under a primer binding site, which may disrupt a primer’s ability to bind. IDT tested the panel with commercially available monkeypox DNA and demonstrated coverage of positions 6760–190,905 (inverted-terminal repeats (ITRs) not included). Data can be generated from extracted viral DNA following a 2.5-hour workflow from viral DNA to libraries. The xGen Monkeypox Viral Amplicon Panel is also compatible with IDT Normalase™ technology.

“As part of IDT’s NGS Tech Access Program, our team was able to acquire the xGen Monkeypox Virus Amplicon Panel and were enthused to see the promising sequencing data it generated for our research,” said Efrem Lim, Ph.D., associate professor at Arizona State University. “Given the importance of understanding infectious disease evolution, we recognize the outstanding work of partners like IDT who leverage their deep genomics expertise to provide cutting-edge NGS solutions that enable research, especially in our public health response to the monkeypox outbreak.”

Want the latest science news straight to your inbox? Become a SelectScience member for free today>>

RUO - For research use only. Not for use in diagnostic procedures. Unless otherwise agreed to in writing, IDT does not intend these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. GMP refers to products manufactured under ISO 13485: 2016 QMS. Purchaser is solely responsible for all decisions regarding the use of these products and any associated regulatory or legal obligations.

Links

Integrated DNA Technologies Inc.Company website

Tags

MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library GenerationLibrary generation refers to the construction of NGS libraries from RNA and DNA sources.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.VirusesViruses are microscopic pathogens that require a host cell to replicate. Understanding their structure, replication cycle, and impact on the immune system is crucial in developing effective treatments, vaccines, and diagnostic methods. Research continues to focus on emerging viral diseases and antiviral drug discovery. Explore solutions for virus research, detection, and treatment in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.

Related articles