Integrated DNA Technologies introduces DsiRNA: high-quality RNA duplexes for RNAi

7 Oct 2008
Samantha Rosoman
Campaign Coordinator

Product news

Integrated DNA Technologies (IDT) has announced the launch of its Screening Dicer-substrate siRNA (DsiRNA) duplex product. Ideal for small scale in vitro applications, Screening DsiRNAs provide researchers with a cost effective and quick solution for their RNAi experiments. All duplexes ship within 4 working days and are QC analyzed by ESI mass spectrometry to verify compound identity.

Produced on IDT’s proprietary high-throughput synthesis platform, the manufacturing process includes Affinity purification and has been designed to provide a high quality product at a low cost. Available in either tubes or plates, IDT offers a 2 or 10 nanomole scale yields. DsiRNA methods were developed in a collaborative project between IDT and Prof. John Rossi at the Beckman Research Institute of the City of Hope National Medical Center.

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OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.
Integrated DNA Technologies introduces DsiRNA: high-quality RNA duplexes for RNAi