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INTEGRA's pipetting solutions speed up viral extraction for COVID-19 testing

INTEGRA Biosciences
9 Jun 2020
Tom Casburn
Associate Editor

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The COVID-19 pandemic has placed unprecedented pressure on clinical research and diagnostic laboratories to process more samples, much more quickly. Time has become one of the most valuable resources for these laboratories, and throughput needs to be significantly increased to handle the greater workload, which many labs do not currently have the capacity to handle. PCR is the method of choice for the identification of acute SARS-CoV-2 infections, as it is both reliable and sensitive. However, the workflow is both costly and labor-intensive, requiring time-consuming extraction of viral RNA, followed by precise mixing of low-volume samples and expensive PCR reagents.

Processing more, faster

One of the first bottlenecks in the workflow for many labs is the large number of patient samples that need to be transferred from nasal/throat swab tubes to microplates or other labware formats prior to RNA extraction for PCR screening. INTEGRA’s VOYAGER adjustable tip spacing pipettes are ideally suited to this task, automatically adjusting tip spacing between different types of labware at the touch of a button. This allows extremely fast, simultaneous reformatting of up to 12 samples from swab tubes to plates, without the errors associated with a single channel pipette. Labs looking to further increase their throughput can even combine the VOYAGER with an ASSIST PLUS pipetting robot, bringing together the speed of multichannel pipetting and the convenience of walk-away automation at a price that almost any lab can afford.

Efficient viral extraction

Once samples are in a microplate format, the compact VIAFLO 96/384 handheld electronic pipette can simultaneously transfer 96 or 384 samples at a time, making it quick and easy to fill whole or partial microplates. This user-friendly solution is well suited to nucleic acid extraction workflows and, together with the VOYAGER and ASSIST PLUS, is compatible with a wide range of extraction methods, including various silica-based kits using vacuum manifolds and most common magnetic bead extraction kits, further increasing throughput at this stage.

Futureproof operation

These easy to use instruments are ideal for laboratories that need to speed up their PCR-based screening workflows to meet urgent COVID-19 demands. Offering the simplicity of a handheld device, plus convenient features to streamline routine tasks, these instruments do not require complex installations or staff to spend time undertaking specialist training or developing new protocols.

Just as importantly, as workflows change and the demand for COVID-19 testing subsides over time, the flexibility of these systems ensures that they will always have a purpose in the lab. From ELISAs and protein purification to sample reformatting and plate replication, INTEGRA pipettes are designed to simplify a wide range of general life sciences and diagnostic tasks.

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VIAFLO 384 – Electronic 24, 96 and 384 Channel Handheld Pipette

INTEGRA Biosciences

INTEGRA Biosciences’ VIAFLO 384 increases productivity of sample preparation in microplates and supports 24, 96 and 384 channel pipetting heads. VIAFLO 384 is used like a standard handheld pipette. It is hand-controlled but movements are supported by motors for an effortless workflow and precise targeting of the microplate wells. With a choice of ten interchangeable pipetting heads, the unit quickly adapts to any 24, 96, 384, or even 1536 well pipetting application in a volume range of 0.5 μl to 1250 μl.  VIAFLO 384 benefits from the innovative GripTip system. The special pipette tips, together with the unique tip fitting, form an integral system. It guarantees tips will not fall off during pipetting and provides a reliable sealing of every tip. VIAFLO 384 can be used for sample transfers from reservoir to plate, plate to plate or within plates. VIAFLO 384 Features and Benefits: VIAFLO 384 is used like a handheld pipette and has an intuitive user interface, freeing staff from training time. Pipetting heads can be changed for an optimal instrument adaption to different volume ranges, increasing accuracy and precision. The innovative GripTip system provides a reliable sealing of every tip and guarantees that tips will never fall off, providing safe and reproducible assay preparation. Various pipetting modes are available for increased productivity and application customization.

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VOYAGER Adjustable Tip Spacing Pipettes

INTEGRA Biosciences

The VOYAGER electronic pipettes enable users to change the tip spacing electronically with the push of a button. Up to three different tip spacings from 4.5 mm to 33 mm can be set and saved. Accelerate reformatting of plates, e.g. 96 well plates to 384 well plates, transfers from racked tubes into microplates or filling pockets of Agarose gels. The VOYAGER electronic pipettes feature a colour display and an intuitive touch wheel user interface. This enables quickest volume changes and easy, ergonomic modification of pipetting parameters. Ten predefined protocols are available, such as Repeat Dispense, Serial Dilute, Sample Mix. In addition, up to 20 customized protocols can be created and stored on the pipette. VOYAGER Adjustable Tip Spacing Pipette Features & Benefits:  Electronically adjustable tip spacing accelerates plate reformatting and sample transfers between different lab ware formats. Unique tip fitting design ensures that tips snap into place and will not accidently fall off. GripTips attach the same way each and every time to enable precise touching off. Intuitive touch wheel interface for rapid volume setting and easy programming. Available Models: 4-channel 6-channel 8-channel 12-channel

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INTEGRA's pipetting solutions speed up viral extraction for COVID-19 testing