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INTEGRA’s pipetting robot supports PCR-based HPV screening across France

INTEGRA Biosciences
16 Feb 2021
Meg Hicks
Student / Graduate

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Several studies have recently suggested that PCR-based screening is more accurate when addressing workloads associated with Human papilloma virus (HPV) than the conventional Papanicolaou (Pap) test and liquid-based cytology. PCR based screening also offers earlier detection of pre-cancerous growths, leading to a gradual shift towards molecular diagnostics approaches.

INTEGRA promise that the ASSIST PLUS pipetting robot is ideally suited to this type of diagnostic screening workflow, offering fast and easy, walkaway automation of protocols. The Centre de Pathologie du Maine Normandie (CPMN) in Le Mans was the first lab in France to install an ASSIST PLUS to process HPV samples, and this system has now been adopted by several labs nationally. Nucleic acid extraction and PCR set-up are combined into a single program on the ASSIST PLUS, significantly simplifying the workflow and ensuring superior reproducibility with error-free pipetting. Dr Quentin Breton, a pathologist at CPMN, commented: “We chose the ASSIST PLUS because we wanted a solution that would allow us to automate the protocol for HPV testing as much as possible, while maintaining strict quality requirements and avoiding pipetting errors. It has now become an integral part of management of our HPV samples, and we have ideas on how to implement it for several other molecular biology workflows.”

CPMN’s ASSIST PLUS is equipped with a VOYAGER adjustable tip spacing pipette, which is used to transfer samples and mastermix from tubes to PCR plate. Maria Falco, Laboratory Technician at CPMN explained: “We use many different labware formats, and precisely and accurately transferring between them is complicated and time consuming to perform manually. Thankfully, the VOYAGER, in combination with the ASISST PLUS, processes these automatically and simultaneously, allowing us to achieve very accurate and reproducible results, and streamlining the entire workflow.”

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VOYAGER Adjustable Tip Spacing Pipettes

INTEGRA Biosciences

The VOYAGER electronic pipettes enable users to change the tip spacing electronically with the push of a button. Up to three different tip spacings from 4.5 mm to 33 mm can be set and saved. Accelerate reformatting of plates, e.g. 96 well plates to 384 well plates, transfers from racked tubes into microplates or filling pockets of Agarose gels. The VOYAGER electronic pipettes feature a colour display and an intuitive touch wheel user interface. This enables quickest volume changes and easy, ergonomic modification of pipetting parameters. Ten predefined protocols are available, such as Repeat Dispense, Serial Dilute, Sample Mix. In addition, up to 20 customized protocols can be created and stored on the pipette. VOYAGER Adjustable Tip Spacing Pipette Features & Benefits:  Electronically adjustable tip spacing accelerates plate reformatting and sample transfers between different lab ware formats. Unique tip fitting design ensures that tips snap into place and will not accidently fall off. GripTips attach the same way each and every time to enable precise touching off. Intuitive touch wheel interface for rapid volume setting and easy programming. Available Models: 4-channel 6-channel 8-channel 12-channel

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic InstrumentsRobotic instruments can be used for high-throughput automation of many lab processes. Such processes use instruments for assays like cell based assays and ELISA, for sample preparation like shakers, centrifuges and incubators, and for analysis such as sequencing and western blot analyzers. Useful features of robotic instruments include speed, reproducibility, barcode readers, software and automation.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automation Supplies / AccessoriesAutomated accessories and consumables make lab processes fast, efficient and reliable. Useful automation accessories include microplate sealers, label printers and reagent reservoirs.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Liquid Handling WorkstationLiquid handling workstations automate the process of sample preparation, improving accuracy and efficiency. These systems are essential for high-throughput applications in biological, chemical, and pharmaceutical research, reducing human error and increasing reproducibility. Workstations come equipped with robotic arms, pipetting tools, and other accessories to streamline lab workflows. Explore liquid handling workstations in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.HPVLab EssentialsLab essentials include laboratory consumables that are vital to the operation of all laboratories.

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