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Intact mass analysis of modified oligonucleotides and impurities using the BioAccord LC-MS System

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Join us on Wednesday, December 2, to learn how the BioAccord LC-MS System delivers robust UPLC separations performance and accurate mass data for a diverse range of modified oligonucleotides

20 Nov 2020
Edward Carter
Publishing / Media

Expert insights

Dr. Catalin Doneanu, Principal Chemist at Waters

As the pipeline for oligonucleotide-based therapeutics continues to grow, with >300 currently in clinical trials, along with an increased demand for GMP/GLP-validated oligonucleotide reagents (including primers, probes, gRNA) for clinical DNA testing/sequencing, the need for robust, compliance-ready LC-MS analysis of oligonucleotides has never been greater.

Waters has designed the BioAccord LC-MS System to deliver robust, reproducible separations and accurate mass performance in a compact, easy-to-use format; one that facilitates deployment across regulated development, manufacturing and QC labs and supports a range of routine biopharmaceutical analyses, including oligonucleotide analysis. In this webinar, hear from Dr. Catalin Doneanu about recent work demonstrating the utility of the BioAccord LC-MS system and find out how this system has helped users analyze and monitor a diverse range of modified oligonucleotides and known impurities using the compliance-ready intact mass analysis workflow.

Register here

Watch this webinar to:

  • Learn how the BioAccord LC-MS system delivers robust UPLC separations performance and accurate mass data for a diverse range of modified oligonucleotides
  • Learn how mass spectral deconvolution algorithms and isotopic models can impact mass accuracy, particularly for phosphorothioated oligonucleotides, and how the BioAccord makes it easy to select the right combination.
  • Discover how compliance-ready data acquisition, processing and reporting take the worry out of meeting data integrity and regulatory requirements.

Who should attend?
This webinar will provide insights for:

  • Lab directors, managers and analytical scientists engaged in oligotherapeutic development, manufacturing, and QC
  • Analytical scientists supporting the development and manufacturing of oligonucleotide primers and probes for PCR-based diagnostics, including targeted next-generation sequencing
  • Incoming QC managers who verify the ID, purity, and quality of incoming oligonucleotides from third-party manufacturers

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

This webinar will run as two sessions, the first session will run on Wednesday, December 2, at:

  • 16:00 GMT
  • 17:00 CET
  • 08:00 PST
  • 11:00 EST

The second session will run on Thursday, December 3, at:

  • 10:30 IST
  • 13:00 CST
  • 14:00 JST

Register for this webinar here>>

SelectScience runs more than 10 webinars a month across various scientific topics, discover more of our webinars here>>

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BioAccord LC-MS System

Waters

The First SmartMS-Enabled Biopharma Solution  The Waters BioAccord System is an integrated system that simplifies high performance LC-MS biopharmaceutical analysis for every user. An easy-to-use system solution that puts the power to make decisions directly in your hands; a self-calibrating, self-optimizing, self-sufficient tool that equips you with high quality data you can use to tackle the challenges you face every day during biopharmaceutical development.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.OligosLC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.

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Intact mass analysis of modified oligonucleotides and impurities using the BioAccord LC-MS System