SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Innovative HP OnGuard siRNA Design ensures successful RNAi

QIAGEN
24 Oct 2006

Product news

HP OnGuard siRNA Design from QIAGEN is an upgrade to the HiPerformance siRNA Design Algorithm. It uses advances in the knowledge of the RNAi mechanism to reduce the risk of off-target effects and increase potency, ensuring successful RNAi.

HP OnGuard siRNA Design incorporates an innovative neural-network algorithm, proprietary homology analysis, and exciting, newly added features. siRNAs are available at QIAGEN’s GeneGlobe™ Web portal via the article webpage.

Design features include 3' UTR/seed region analysis, SNP avoidance, and interferon motif avoidance. siRNA design is reinforced using the results from QIAGEN’s siRNA validation project in which thousands of siRNAs have been tested for effectiveness by real-time RT-PCR. Data from Affymetrix® GeneChip® analysis is used to minimize the potential for off-target effects.

As well as siRNAs designed using HP OnGuard siRNA Design, matching RT-PCR assays, RNAi controls, and products for transfection and downstream analysis are available at GeneGlobe, covering the entire RNAi workflow.

Links

QIAGENCompany website

Tags

PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Chem / BioinformaticsCheminformatics and bioinformatics are computational techniques used in chemistry and biology, respectively, for data acquisition, processing and storage. Cheminformatics focuses on compound information, whereas bioinformatics is mainly applied to analysis and modeling of genomics, genetic and sequencing information. Hardware and software is available for data acquisition, analysis, management and storage.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

Related articles

Innovative HP OnGuard siRNA Design ensures successful RNAi