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Innovation: TF Chip Stem Cell kit- a new tool for stem cell characterization

Eppendorf
13 Sept 2007

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The TF Chip Stem Cell kit enables profiling the status of a stem cell culture by 12 specific transcription factors in one assay. Two identical arrays are spotted on one slide and allow a direct comparison of two samples treated differently.

Double-stranded DNA containing the transcription factor specific binding sequence is spotted onto a coated glass surface. Mutated sequences serve as negative binding controls. The array is incubated with a nuclear protein extract and transcription factor proteins are monitored with antibodies specific for their activated status. Detection can be done with Silverquant® technology or with fluorescence. This kit enables the analysis of the status of a stem cell culture in just one experiment in a convenient and simple workflow. It can be used for human, mouse or rat samples.

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Lab-on-a-ChipLab-on-a-chip (LOC) technology uses microfluidic chips, such as microarrays, to perform biochemical assays on a small scale. Benefits of lab-on-a-chip include the small volume of sample required as well as the speed of analysis. In selecting lab-on-a-chip devices consider the assay, cost, reliability, sensitivity and signal to noise ratio.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microarray AnalysisMicroarrays, also known as biochips, are used for the detection and analysis of multiple genes, proteins, antibodies, or biomarkers on a single microchip. This can reveal information on protein or gene expression, single nucleotide polymorphism (SNP), copy number variation (CNV), epigenetics and patient health in clinical diagnostic tests. Discover a range of microarray scanners and prefabricated antibody, protein, RNA and DNA microarrays for your analysis or consider creating your own custom microarrays with a microarray printer. Find the best microarray products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Innovation: TF Chip Stem Cell kit- a new tool for stem cell characterization