Improve Speed and Sensitivity for your LC/MS Analysis

8 May 2008
Greg Smith
Analyst / Analytical Chemist

Product news

Phenomenex presents HPLC options for increasing lab productivity at ASMS 2008. Visit booth #131 to see new products for LC/MS techniques and applications. Poster presentations will introduce Phenomenex research into the analysis of drug compounds in biomatrices and the effect of pH on biological samples in RP LC-ESI+-MS/MS.

Ideal Columns for LC/MS Applications – Booth #131

Jupiter® 300 3µ
Ideal for separating closely eluting proteins, Jupiter 300 3µ columns give better resolution and sharper peaks than other phases. These columns fully resolve post-translational modifications and are ideal for research on proteins such as ctytochrome C and beta-lactoglobulin.

Gemini®-NX
Through a second-generation organo-silica grafting process, Gemini-NX is a rugged column platform that simplifies column selection and enables robust method development for ionizable compounds. One of the most versatile reversed phase columns from Phenomenex, Gemini-NX excels in both MS-volatile buffers and non-volatile buffers, offers high sample loading capacity, and is one of the most durable, pH tolerant columns on the market.

Onyx™ 2.0 mm ID Monolithic
Now you can achieve sample throughput at monolithic speeds! The new 50 mm x 2.0 mm ID format offers solutions for faster sample throughput at low pressures and high efficiencies.

Poster Presentations

• Ideal Sample Preparation and HPLC Column Choice for the Analysis of Drug Compounds in Biomatrices (M, 6/02, 11:15 - 3:30)

• Effect of pH on the Quantification and Ion Suppression of Basic Drugs in Samples of Biological Origin by RP LC-ESI+-MS/MS (M, 6/02, 11:15 - 3:30)

Onyx Monolithic LC Columns

Phenomenex Inc

Acheive Faster HPLCOnyx monolithic silica-based HPLC columns are designed for high-throughput analysis and the direct injection of dirty or viscous samples. Reduce run times with extremely high flow rates (up to 9 mL/min) "Dilute-and-Shoot” dirty biological samples Scalable from capillary to semi-prep column dimensions Onyx columns deliver high efficiency separations and can be coupled together in series to produce the extreme plate counts needed to separate very complex mixtures.Dilute & Shoot Complex mixtures coupled with dirty matrices, such as serum samples encountered in the pharmaceutical/biotechnology industry, are quite difficult and time-consuming to analyze. With Onyx however, you have the option to dilute-and-shoot your sample directly onto the column because it won't clog due to the macroporous system or cause high backpressures. Flow Rate Flexibility Another unique feature is the ability to use flow rate as parameter for optimizing separations. Onyx columns are responsive to changes in flow rate and can be changed mid run to reduce total run time when certain compounds elute much later. This type of flow rate flexibility gives many options for optimization.

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Jupiter LC Columns for Proteins & Peptides

Phenomenex Inc

RP-HPLC for Protein/Peptide Analysis and PurificationJupiter® HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification. A wide variety of phases (C18, C12, C5, C4) and dimensions (capillary-preparative)/bulk media are available to support the majority of separation requirements.Jupiter 300 - 300 Å columns designed to purify intact proteinsJuptier Proteo - 90 Å columns engineered for increased peak capacity and resolution of peptide maps as well as peptide separationsExplore Aeris Core-Shell HPLC/UHPLC columns for ultra-high performance protein/peptide analysis and characterization.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.
Improve Speed and Sensitivity for your LC/MS Analysis