IDT showcases latest technologies at Biotechnica 2008

30 Sept 2008
Kerry Parker
CEO

Product news

Integrated DNA Technologies (IDT), a worldwide leader in custom DNA and RNA synthesis, will be presenting a range of recently launched products and services in hall 9, stand C58 at Biotechnica 2008. These include the Screening Dicer-substrate RNAi (DsRNAi) for cost-effective siRNA research; TriFECTinTM for introducing short oligos into primary or immortalized cell lines; and the convenient SameDay® Oligo service, saving researchers valuable time.

DsRNAi duplexes all undergo strict ESI mass spectrometry, ensuring that both sense and antisense RNA strands have been correctly synthesized. Furthermore, the proprietary cationic lipid formulation, TriFECTin, has been optimized to deliver DsRNAi and anti-mRNA antisense oligos, maximizing the success of RNAi-directed gene silencing and miRNA knockdowns. Any unmodified standard oligo ordered before midday (GMT) can be dispatched the same day to arrive with the researcher the very next morning, using the SameDay Oligo Service.

Dr. Brian Sproat will elaborate on this information in his Innovative Forum presentation on October 7th, entitled “Synthesis and application of dicer-substrate RNA duplexes.” For further information, two press releases are available on the Biotechnica website or for details of the full range of products and services, please visit the company website.

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OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.