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IDT launches rhAmpSeq™ CRISPR analysis system

Integrated DNA Technologies Inc.

New system provides end-to-end solution for researchers to validate specificity of CRISPR and other genome editing projects

28 Mar 2021
Diane Li
Assistant Editor

Product news

Integrated DNA Technologies (IDT), a leading comprehensive genomics solutions provider, has launched its rhAmpSeq™ CRISPR Analysis System, which offers an end-to-end solution for characterizing and quantifying the full array of on- and off-target genome editing events in CRISPR research projects.

The system harnesses the power of proprietary IDT rhAmpSeq technology, which enables highly accurate amplicon sequencing on Illumina next generation sequencing (NGS) platforms. The rhAmpSeq CRISPR Analysis System consists of design and synthesis of a custom rhAmpSeq CRISPR Panel, an efficient library kit, and access to data analysis through a user-friendly interface. This system offers a convenient workflow that enables sample-to-sequencer-to-results in less than a week.

“This new system combines IDT’s leading Alt-R™ CRISPR genome editing reagents and innovative data analysis technology to provide researchers a complete solution,” said Trey Martin, IDT President. “As genome editing becomes more widespread, it is crucial to validate the editing and identify off-target effects. The rhAmpSeq CRISPR Analysis System addresses and uniquely enables this important researcher need.”

The system includes IDT’s rhAmpSeq CRISPR Analysis Tool, a cloud-based data analysis pipeline that produces publication-ready graphs and figures, helping researchers eliminate the need for bioinformatics resources or expertise. This comprehensive analysis tool is hosted by BlueBee, an Illumina company. BlueBee is a customizable, cloud-based data platform company that provides genomics analysis solutions.

“Bioinformatics is a vital expertise that many labs do not have available. The rhAmpSeq CRISPR Analysis System helps to fill that gap and streamline workflows to get end results quickly and efficiently,” added Martin. “BlueBee is a valuable partner for IDT as their cloud-based genomics analysis solutions provide a secure, robust, scalable, and user-friendly platform for research.”

“Illumina has a great history of partnership with IDT. We are pleased to extend this partnership and together provide researchers with an unparalleled ability to accelerate genome editing projects by translating raw sequencing data into human-readable information,” said Hans Cobben, Vice President of Software Development at Illumina.

Validation of specificity for CRISPR genome editing can be particularly beneficial for research projects that require detailed on- and off-target data analysis. The validation is also valuable for drug target discovery and for labs publishing in high impact journals. Off-target effects may have unwanted consequences; minimizing the risks associated with off-target genome editing is therefore essential.

The rhAmpSeq CRISPR Analysis System is the latest addition to IDT’s expanding portfolio of Alt-R CRISPR genome editing products designed to meet the needs of CRISPR researchers. IDT’s product line offers a complete workflow – from design to analysis – to enable research projects through quality, simplicity, and cost efficiency. The Alt-R CRISPR portfolio is popular amongst many top academic researchers, as well as biotech and pharmaceutical companies.

Do you use Integrated DNA Technologies (IDT) products in your lab? Write a review today for your chance to win a $400 Amazon Gift Card>>

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Epigenetic AnalysisEpigenetic analysis products are used to study heritable phenotype changes that do not alter the DNA sequence, such as DNA modifications affecting gene activity. Determine whether genes have been methylated with DNA methylation quantification kits or methylation microarrays. Assess epigenetic regulation with histone modification kits or ChIP-on-chip microarrays. Kits are also available for bisulfite conversion and amplification of your samples, as well as epigenomic library preparation kits for next-generation sequencing (NGS). Find the best epigenetic products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Nucleic Acid ConcentrationGene EditingGene editing is the precise alteration to an organism's DNA, with CRISPR being the most well-known technique. It is used in a wide range of applications, including the development of disease models and gene therapy, and crop improvement. Browse our peer-reviewed product directory to find the best gene editing solutions, compare products, check reviews, and get pricing directly from manufacturers.Genome EditingGenome editing involves altering the DNA of an organism, which holds promise for disease treatment, agriculture, and gene therapy. Techniques like CRISPR-Cas9 are revolutionizing this field, enabling precise genetic modifications. Explore genome editing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.Genome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.CRISPRCRISPR technology enables precise editing of genes, allowing scientists to modify DNA at specific locations. This revolutionary tool is used in genetic research, drug development, and gene therapy. CRISPR has applications in agriculture, disease treatment, and creating genetically modified organisms (GMOs). Explore CRISPR solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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