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IDT introduces miRCat™ small RNA cloning system

Integrated DNA Technologies Inc.
17 Jul 2007

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Integrated DNA Technologies (IDT) has today announced the availability of its new proprietary miRCat™ small RNA cloning system. The unique kit-based resources make the creation of small RNA (including miRNAs, piRNAs and endogenous siRNAs) libraries from any primary RNA source, highly time and cost efficient with peerless flexibility and consistency. The new miRCat system permits cloning of very rare small RNAs and takes into account the natural variability in structure and sequence between species. Conveniently, it is compatible with most existing standard laboratory protocols for processes such as RNA extraction, purification and cloning. These features make the miRCat small RNA cloning system the ideal solution for all small RNA research.

Based upon a pre-activated adenylated oligonucleotide linkering method, the miRCat cloning system has been carefully designed to make small RNA library creation easy for all researchers. It consists of three sequential protocols: RNA isolation and enrichment, followed by cloning linker attachment, and ending with an amplification and cloning phase. The system can be used for concatamer cloning or direct ‘shotgun’ cloning; it also can provide PCR amplicons suitable for use with TOPO TA Cloning®* or pGEM® T-Easy# cloning vectors.

For cloning small RNAs lacking the 5’-phosphorylated end present in miRNAs, a modified kit - miRCat-33™ - has been developed, which uses 5’ ligation-independent cloning. This makes it an excellent cloning system for the recently discovered C. elegans 5’ tri-phosphorylated microRNAs, which would otherwise be omitted. Furthermore, optional kit components ensure additional versatility with a choice of three different 3’ cloning linkers. These provide various restriction site combinations to fit with any preferred cloning processes. Specific primers are also available to convert the resultant small RNA libraries using Roche/454 Life Sciences sequencing systems.

* TOPO TA Cloning is a registered trademark of Invitrogen Corporation
# pGEM® T-Easy is a registered trademark Promega Corporation

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Compound LibrariesCompound libraries, or chemical libraries, are used in drug discovery for the identification of potential therapeutics compounds. Used in conjunction with high-throughput screening, the libraries of stored compounds are often generated for specific purposes as a drug target or disease model. Cheminformatics are commonly used when designing a compound library and software can be used to analyze the screening process.  DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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IDT introduces miRCat™ small RNA cloning system