ICT Introduces Two New ELISA Blocking Buffer Formulations

26 May 2014

Sarah Thomas

Associate Editor

Product news

Proper blocking of the unoccupied areas of the ELISA plate wells is paramount to reducing background noise and attaining an accurate result. The two new formulations offer researchers additional choices in reagents to optimize their ELISAs.

ICT's ELISA Blocking Buffer formulations reduce nonspecific binding of sample and assay components to the ELISA well while stabilizing the coated protein. The Alternative Block Blocking Buffer (Code BB5) is a new all-synthetic ELISA blocking buffer free of proteins and detergents. The Monster Block Blocking Buffer (Code BB6) is a new ELISA blocking buffer utilizing a heterogenous mixture of non-mammalian blocking agents.

ICT's ELISA Blocking Buffer formulations reduce nonspecific binding of sample and assay components to the ELISA well while stabilizing the coated protein. Six formulations offer various benefits to different assay situations - General Blocker Blocking Buffer BB1, Neptune Block Blocking Buffer BB2, SynBlock Blocking Buffer BB3, Phosph-Free Blocking Buffer BB4, Alternative Block Blocking Buffer BB5, and Monster Block Blocking Buffer BB6. ICT also offers a Blocking Buffer Optimization Pack (BBOP), which allows researchers to try three different formulations at a reduced rate.

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Alternative Block ELISA Blocking Buffer
Immunochemistry Technologies LLC
Reduce non specific binding in ELISAs and stabilize proteins with Alternative Block, an all-synthetic ELISA blocking buffer free of proteins and detergents. Alternative Block is a protein-free, detergent-free ELISA blocking buffer. This unique blocking buffer contains a heterogeneous mixture of proprietary blockers and synthetic stabilizers that block the uncoated regions of the microtiter plate without the use of conventional cross-reactive protein additives or detergents.Alternative Block's all-synthetic heterogenous mixture of blocking agents minimizes non specific binding interactions during the immunoassay process. Alternative Block provides a microhydrated environment to stabilize the coated protein during long-term storage for improved retention of antigen epitope and antibody binding activity.Features: Reduces non specific binding interactions through the use of an all-synthetic mixture of blocking agents Stabilizes dried, coated protein by providing a microhydrated environment for better retention of antigen epitope and antibody binding activity Beneficial for all ELISA formats Allows for long-term storage of manufactured plate batches
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General Block ELISA Blocking Buffer
Immunochemistry Technologies LLC
General Block contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide or protein antigen-down ELISA formats. General Block contains a mammalian protein blocking agent designed to reduce nonspecific background noise in most antibody capture ELISA formats and peptide/protein antigen-down ELISA formats. This unique, low-level blocking buffer minimizes nonspecific binding interactions during the immunoassay process.General Block contains proprietary protein stabilizers that provide a long-term stabilizing environment for dried antigen or antibody coat proteins. Stabilization of the coated protein during short- or long-term storage results in better binding reactivity during the immunoassay, reducing the amount of necessary coat protein and increasing assay shelf life.Features: Provides a long-term stabilizing environment for dried antigen or antibody coat proteins Minimizes nonspecific binding interactions during the assay process Compatible with antibody capture (sandwich) and antigen-down ELISA formats Suitable for room temperature blocking and long-term dried plate storage at 2°-8°C Supplied at 1X
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Monster Block ELISA Blocking Buffer
Immunochemistry Technologies LLC
Reduce non specific binding in ELISAs, address high background issues, and stabilize proteins with Monster Block, a new ELISA blocking buffer utilizing a heterogenous mixture of nonmammalian blocking agents. Monster Block™ provides a high degree of blocking efficiency and protein stabilization through the use of non-mammalian protein blocking agents and stabilizers. The heterogeneous composition of this unique ELISA blocking buffer efficiently blocks the uncoated regions of the microtiter plate without the use of mammalian protein additives.Monster Block's heterogenous mixture of nonmammalian blocking agents minimizes non specific binding interactions during the immunoassay process, reducing ELISA background noise and increasing specific signal. In addition, the non-mammalian-based Monster Block formulation is antigenically foreign to most mammalian immune systems, reducing the chance of cross-reactivity with anti-isotype detection antibodies.Features: High degree of blocking efficiency to address high background problems in antigen-down and sandwich immunoassays Beneficial for all ELISA formats Useful for testing mammalian serum samples: Nonmammalian formulation reduces risk of endogenous antibodies in sample reacting with adsorbed protein Stabilizes coated protein during long-term storage by providing a microhydrated environment for improved retention of antigen epitope and antibody binding activity
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Neptune Block ELISA Blocking Buffer
Immunochemistry Technologies LLC
Neptune Block Blocking Buffer is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. Neptune Block ELISA Blocking Buffer is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.Neptune Block ELISA Blocking Buffer is a mixture of small molecular proteins and molecular stabilizers capable of blocking the nonspecific binding sites of the adsorbed proteins, as well as the unoccupied regions of the plate that are not accessible to larger mammalian blocking reagents. When used in antigen-down ELISA formats, Neptune Block's low molecular-weight proteins reduce the possibility of false-positives generated from endogenous antibodies in the sample reacting with plate-blocking proteins.Features: Utilizes non-mammalian protein blocking molecules Heterogenous formulation blocks smaller unoccupied regions of the plate Increases signal to background ratio Stabilizes adsorbed proteins for maximum binding capability Ready to use
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Phosph-Free Block ELISA Blocking Buffer
Immunochemistry Technologies LLC
Phosph-Free Block ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs.Phosph-Free Block is formulated for ELISAs using alkaline phosphatase detection or for assays with ultra-sensitivity requirements. However, it is appropriate for assays using HRP detection as well. Using inert non-reactive blockers, this synthetic blocking buffer reduces the amount of nonspecific binding of enzyme labeled conjugates to blocked plate surfaces. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.
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SynBlock ELISA Blocking Buffer
Immunochemistry Technologies LLC
Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. SynBlock ELISA Blocking Buffer provides superior background-blocking performance without the use of conventional cross-reactive protein additives. SynBlock ELISA Blocking Buffer is a novel non-protein blocking formulation designed to eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. Use of this blocking formulation provides superior background performance without the use of conventional cross-reactive protein additives.Containing inert non-reactive blockers, SynBlock Blocking Buffer reduces the amount of nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces. When blocking the coated immunoassay plate wells, SynBlock ELISA Blocking Buffer provides a microhydrated environment to stabilize the coated protein for better binding reactivity during the immunoassay and during long-term storage.Features: All synthetic blocking formulation; protein-free Reduces nonspecific binding of enzyme-labeled conjugates to blocked plate surfaces Stability enables dried plates to be stored for up to 18 months. Supplied at 1X
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